Evidence map›Paper›PMID 29335561›Full record

ArticleNature structural & molecular biology2018

Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp.

Marcus Gallagher-Jones, Calina Glynn, David R Boyer, Michael W Martynowycz, Evelyn Hernandez, Jennifer Miao, Chih-Te Zee, Irina V Novikova, Lukasz Goldschmidt, Heather T McFarlane and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature structural & molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.

0numbers the graph read from it
0cells of the map it votes in
54citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

54 citing papers in PubMed.

  1. Article
  2. Article
  3. Backbone double-mutant cycle analysis quantifies hydrogen-bond energies in proteins.Protein science : a publication of the Protein Society · 2026
    Article
  4. Article
  5. Unraveling atomic complexity from frozen samples.Structural dynamics (Melville, N.Y.) · 2025
    Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Fragment-BasedACS bio & med chem Au · 2023
    Article
  11. MicroED Structure of a Protoglobin Reactive Carbene Intermediate.Journal of the American Chemical Society · 2023
    Article
  12. MicroED in drug discovery.Current opinion in structural biology · 2023
    Review
  13. Review
  14. Structural biology of ex vivo mammalian prions.The Journal of biological chemistry · 2022
    Review
  15. Article
  16. Review
  17. Article
  18. Article
  19. Review
  20. MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Marcus Gallagher-JonesDepartment of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, University of California Los Angeles, Los Angeles, CA, USA.
Calina GlynnDepartment of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, University of California Los Angeles, Los Angeles, CA, USA.
David R BoyerDepartment of Biological Chemistry and Department of Chemistry and Biochemistry, University of California Los Angeles, Howard Hughes Medical Institute, UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, CA, USA.
Michael W MartynowyczJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Evelyn HernandezDepartment of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, University of California Los Angeles, Los Angeles, CA, USA.
Jennifer MiaoDepartment of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, University of California Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0001-5527-295X
Chih-Te ZeeDepartment of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, University of California Los Angeles, Los Angeles, CA, USA.
Irina V NovikovaEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
Lukasz GoldschmidtDepartment of Biological Chemistry and Department of Chemistry and Biochemistry, University of California Los Angeles, Howard Hughes Medical Institute, UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, CA, USA.
Heather T McFarlaneDepartment of Biological Chemistry and Department of Chemistry and Biochemistry, University of California Los Angeles, Howard Hughes Medical Institute, UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, CA, USA.
Gustavo F HelgueraLaboratory of Pharmaceutical Biotechnology, Institute of Biology and Experimental Medicine, Buenos Aires, Argentina.
James E EvansEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
Michael R SawayaDepartment of Biological Chemistry and Department of Chemistry and Biochemistry, University of California Los Angeles, Howard Hughes Medical Institute, UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, CA, USA.
Duilio CascioDepartment of Biological Chemistry and Department of Chemistry and Biochemistry, University of California Los Angeles, Howard Hughes Medical Institute, UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, CA, USA.
David S EisenbergDepartment of Biological Chemistry and Department of Chemistry and Biochemistry, University of California Los Angeles, Howard Hughes Medical Institute, UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0003-2432-5419
Tamir GonenJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Jose A RodriguezDepartment of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, University of California Los Angeles, Los Angeles, CA, USA. jrodriguez@mbi.ucla.edu.ORCID http://orcid.org/0000-0001-8471-2504

Funding

Overall EvaluationP41GM103403 · NIGMS · CORNELL UNIVERSITY · PI EALICK, STEVEN E · 2012 to 2017
$14.3M
UCLA MSD ProgramR25GM055052 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BARBER, PAUL HENRY, HASSON, TAMA W. · 1996 to 2021
$9.2M
Structural Biology of Amyloid DiseaseR01AG029430 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI EISENBERG, DAVID · 2007 to 2016
$3.9M
Micro Electron Diffraction of Toxic and/or Infectious Macromolecular NanoassembliesR35GM128867 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Jose Alfonso Rodriguez · 2018 to 2026
$3.1M
NIA NIH HHS R01 AG029430NIGMS NIH HHS P41 GM103403NIGMS NIH HHS R25 GM055052NIGMS NIH HHS R35 GM128867
6 · The paper itself

Abstract

The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-ångström-resolution structure of a protofibril formed by a wild-type segment from the β2-α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named 'polar clasps'.

Indexed as

Cryoelectron MicroscopyHydrogen BondingAmyloidAmyloidogenic ProteinsAnimalsCarbamazepineCattleCricetinaeDeerElectronsHumansMicePeptidesPhylogenyPrionsProtein Structure, SecondaryAmyloidAmyloidogenic ProteinsCarbamazepinePeptidesPrionsProteome

Identifiers

PMID29335561
PMCPMC6170007

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.