ArticleNature communications2018
Pixelated spatial gene expression analysis from tissue.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Versatility of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) from diagnosis of early pathological infection to mutation detection in organisms.Molecular biology reports · 2024Review
- Quantitative mRNA expression measurement at home.Scientific reports · 2024Article
- A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A new paradigm for leprosy diagnosis based on host gene expression.PLoS pathogens · 2021Article
- Three-dimensional microscale hanging drop arrays with geometric control for drug screening and live tissue imaging.Science advances · 2021Article
- Rapid isothermal amplification and portable detection system for SARS-CoV-2.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2.bioRxiv : the preprint server for biology · 2020Article
- The Spatial and Genomic Hierarchy of Tumor Ecosystems Revealed by Single-Cell Technologies.Trends in cancer · 2019Review
- Article
- Single-Cell RNA Sequencing-Based Computational Analysis to Describe Disease Heterogeneity.Frontiers in genetics · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 4 institutions in 1 country.
Funding
Abstract
Here, we present a technique that performs on-chip picoliter real-time reverse transcriptase loop mediated isothermal amplification (RT-LAMP) reactions on a histological tissue section without any analyte purification while preserving the native spatial location of the nucleic acid molecules. We demonstrate this method by amplifying TOP2A messenger RNA (mRNA) in a prostate cancer xenograft with 100 µm spatial resolution and by visualizing the variation in threshold time of amplification across the tissue. The on-chip reaction was validated by mRNA fluorescence in situ hybridization (mFISH) from cells in the tissue section. The entire process, from tissue loading on microchip to results from RT-LAMP can be carried out in less than 2 h. We anticipate that this technique, with its ease of use, fast turnaround, and quantitative molecular outputs, would become an invaluable tissue analysis tool for researchers and clinicians in the biomedical arena.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.