Evidence map›Paper›PMID 29335281›Full record

ArticleThe EMBO journal2018

High-throughput identification of RNA nuclear enrichment sequences.

Chinmay J Shukla, Alexandra L McCorkindale, Chiara Gerhardinger, Keegan D Korthauer, Moran N Cabili, David M Shechner, Rafael A Irizarry, Philipp G Maass, John L Rinn

Abstract read
In one paragraph

Article in The EMBO journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed.

  1. Article
  2. Article
  3. Robust mammalian RNA localization elements are complex and multipartite.bioRxiv : the preprint server for biology · 2026
    Article
  4. Review
  5. High-throughput Screening of Sequence Elements Associated with RNA Localization.Computational and structural biotechnology journal · 2026
    Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Review
  11. Review
  12. Article
  13. Review
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Article
  20. Review

14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Chinmay J ShuklaDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Alexandra L McCorkindaleDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Chiara GerhardingerDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Keegan D KorthauerDepartment of Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA, USA.ORCID 0000-0002-4565-1654
Moran N CabiliBroad Institute of MIT and Harvard, Cambridge, MA, USA.
David M ShechnerDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Rafael A IrizarryDepartment of Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
Philipp G MaassDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.ORCID 0000-0002-2742-8301
John L RinnDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA john.rinn@colorado.edu.ORCID 0000-0002-7231-7539

Funding

Regulatory Roles and Dynamics of Nuclear long-noncoding RNAs in PluripotencyP01GM099117 · NIGMS · HARVARD UNIVERSITY · PI MEISSNER, ALEXANDER · 2011 to 2021
$21.2M
Preprocessing and Analysis Tools for High-Throughput TechnologiesR01GM083084 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI IRIZARRY, RAFAEL ANGEL · 2007 to 2018
$4.4M
Deciphering the function and mechanisms of lncRNA-mediated organization of nuclear compartmentsU01DA040612 · NIDA · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI GUTTMAN, MITCHELL, RINN, JOHN LOUIS · 2015 to 2019
$4.1M
Overcoming bias and unwanted variability in next generation sequencingR01HG005220 · NHGRI · JOHNS HOPKINS UNIVERSITY · PI IRIZARRY, RAFAEL ANGEL · 2010 to 2018
$3.6M
Functional Roles of Long Noncoding RNAs During Neuronal DevelopmentR01MH102416 · NIMH · HARVARD UNIVERSITY · PI ARLOTTA, PAOLA · 2014 to 2018
$2.1M
NHGRI NIH HHS R01 HG005220NIDA NIH HHS U01 DA040612NIGMS NIH HHS P01 GM099117NIGMS NIH HHS R01 GM083084NIMH NIH HHS R01 MH102416
6 · The paper itself

Abstract

In the post-genomic era, thousands of putative noncoding regulatory regions have been identified, such as enhancers, promoters, long noncoding RNAs (lncRNAs), and a cadre of small peptides. These ever-growing catalogs require high-throughput assays to test their functionality at scale. Massively parallel reporter assays have greatly enhanced the understanding of noncoding DNA elements

Indexed as

Cell NucleusHeLa CellsHigh-Throughput Nucleotide SequencingHumansIn Situ Hybridization, FluorescenceRNA, Long NoncodingSequence Analysis, RNARNA, Long Noncodingde novo inference of regionshigh‐throughput reporter assaylncRNAnuclear localizationRNA

Identifiers

PMID29335281
PMCPMC5852646

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.