ArticleNature chemical biology2018
A de novo enzyme catalyzes a life-sustaining reaction in Escherichia coli.
Article in Nature chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- De novo gene birth and the conundrum of ORFan genes in bacteria.Genome research · 2025Review
- Article
- Spiers Memorial Lecture: Engineering biocatalysts.Faraday discussions · 2024Article
- Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins.Nature chemistry · 2024Article
- Evolution and synthetic biology.Current opinion in microbiology · 2023Review
- From random to rational: improving enzyme design through electric fields, second coordination sphere interactions, and conformational dynamics.Chemical science · 2023Review
- Crystal structure and activity of a de novo enzyme, ferric enterobactin esterase Syn-F4.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Protection of Catalytic Cofactors by Polypeptides as a Driver for the Emergence of Primordial Enzymes.Molecular biology and evolution · 2023Article
- A de novo protein catalyzes the synthesis of semiconductor quantum dots.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Correlation coefficient-directed label-free characterization of native proteins by surface-enhanced Raman spectroscopy.Chemical science · 2022Article
- From peptides to proteins: coiled-coil tetramers to single-chain 4-helix bundles.Chemical science · 2022Article
- Toward Microbial Recycling and Upcycling of Plastics: Prospects and Challenges.Frontiers in microbiology · 2022Review
- De novo proteins from random sequences through in vitro evolution.Current opinion in structural biology · 2021Review
- CoLiDe: Combinatorial Library Design tool for probing protein sequence space.Bioinformatics (Oxford, England) · 2021Article
- Driving forces in the origins of life.Open biology · 2021Article
- Catalytic Principles from Natural Enzymes and Translational Design Strategies for Synthetic Catalysts.ACS central science · 2021Review
- Systematic engineering of artificial metalloenzymes for new-to-nature reactions.Science advances · 2021Article
- Yeast-Based Biosensors: Current Applications and New Developments.Biosensors · 2020Review
- A Strategy for Combinatorial Cavity Design in De Novo Proteins.Life (Basel, Switzerland) · 2020Article
- A de novo peroxidase is also a promiscuous yet stereoselective carbene transferase.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Producing novel enzymes that are catalytically active in vitro and biologically functional in vivo is a key goal of synthetic biology. Here we describe Syn-F4, the first de novo protein that meets both criteria. Purified Syn-F4 hydrolyzes the siderophore ferric enterobactin, and expression of Syn-F4 allows an inviable strain of Escherichia coli to grow in iron-limited medium. These findings demonstrate that entirely new sequences can provide life-sustaining enzymatic functions in living organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.