ArticleScientific reports2018
Detection of Apparent Cell-free M. tuberculosis DNA from Plasma.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA for tuberculosis: A systematic review and meta-analysis.PloS one · 2021Pooled it
- Role of cell-free DNA technology in assisting paraffin-embedded cytology for the pathological diagnosis of pulmonary tuberculosis.BMC pulmonary medicine · 2026Article
- PlasmaInfectious diseases (London, England) · 2025Article
- MultigeneThe international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease · 2025Article
- Cell-free DNA blood test for the diagnosis of pediatric tuberculous meningitis.Journal of clinical tuberculosis and other mycobacterial diseases · 2024Article
- Genome-wide tiled detection of circulating Mycobacterium tuberculosis cell-free DNA using Cas13.Nature communications · 2023Article
- Time-series prediction and detection of potential pathogens in bloodstream infection using mcfDNA sequencing.Frontiers in cellular and infection microbiology · 2023Article
- Pediatric Tuberculosis Diagnostics: Present and Future.Journal of the Pediatric Infectious Diseases Society · 2022Article
- Gene-Based Diagnosis of Tuberculosis from Oral Swabs with a New Generation Pathogen Enrichment Technique.Microbiology spectrum · 2022Article
- Diagnosing Pulmonary Tuberculosis by Using Sequence-Specific Purification of Urine Cell-Free DNA.Journal of clinical microbiology · 2021Article
- Detection ofThe international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease · 2021Article
- Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR.Folia microbiologica · 2021Article
- Article
- One-Step Nucleic Acid Purification and Noise-Resistant Polymerase Chain Reaction by Electrokinetic Concentration for Ultralow-Abundance Nucleic Acid Detection.Angewandte Chemie (International ed. in English) · 2020Article
- Investigation of Preanalytical Variables Impacting Pathogen Cell-Free DNA in Blood and Urine.Journal of clinical microbiology · 2019Article
- Toward the Development of a Circulating Free DNA-BasedJournal of clinical microbiology · 2019Review
- Tubercular DNA PCR of ocular fluids and blood in cases of presumed ocular tuberculosis: a pilot study.Therapeutic advances in ophthalmologyArticle
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
New diagnostics are needed to improve clinicians' ability to detect tuberculosis (TB) disease in key populations such as children and persons living with HIV and to rapidly detect drug resistance. Circulating cell-free DNA (ccfDNA) in plasma is a diagnostic target in new obstetric and oncologic applications, but its utility for diagnosing TB is not known. Here we show that Mycobacterium tuberculosis complex DNA can be detected in plasma of persons with sputum smear-positive TB, even in the absence of mycobacteremia. Among 40 participants with bacteriologically-confirmed smear-positive TB disease who had plasma tested by quantitative PCR (qPCR), 18/40 (45%) had a positive result on at least one triplicate reaction. Our results suggest that plasma DNA may be a useful target for improving clinicians' ability to diagnose TB. We anticipate these findings to be the starting point for optimized methods of TB ccfDNA testing and sequence-based diagnostic applications such as molecular detection of drug resistance.
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