ArticleEMBO reports2018
Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment.
Article in EMBO reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Spindle morphology changes between meiosis and mitosis driven by CK2 regulation of the Ran pathway.The Journal of cell biology · 2025Article
- The Kinesin-14 tail: Dual microtubule binding domains drive spindle morphogenesis through tight microtubule cross-linking and robust sliding.Molecular biology of the cell · 2025Article
- Force-transducing molecular ensembles at growing microtubule tips control mitotic spindle size.Nature communications · 2024Article
- Effects of Exogenous Regulation of PPARγ on Ovine Oocyte Maturation and Embryonic Development In Vitro.Veterinary sciences · 2024Article
- How Do Environmental Toxicants Affect Oocyte Maturation Via Oxidative Stress?Advances in anatomy, embryology, and cell biology · 2024Review
- Cross-linker design determines microtubule network organization by opposing motors.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Article
- Acentriolar spindle assembly in mammalian female meiosis and the consequences of its perturbations on human reproduction†.Biology of reproduction · 2022Review
- Review
- Stable kinetochore-microtubule attachments restrict MTOC position and spindle elongation in oocytes.EMBO reports · 2021Article
- Nampt-mediated spindle sizing secures a post-anaphase increase in spindle speed required for extreme asymmetry.Nature communications · 2020Article
- Kinesin-14 family proteins and microtubule dynamics defineJournal of cell science · 2020Article
- Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes.Nature communications · 2020Article
- Artificially decreasing cortical tension generates aneuploidy in mouse oocytes.Nature communications · 2020Article
- Mechanisms underlying disruption of oocyte spindle stability by bisphenol compounds.Reproduction (Cambridge, England) · 2020Article
- The mechanisms and clinical application of mosaicism in preimplantation embryos.Journal of assisted reproduction and genetics · 2020Review
- NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells.The EMBO journal · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitotic spindles assemble from two centrosomes, which are major microtubule-organizing centers (MTOCs) that contain centrioles. Meiotic spindles in oocytes, however, lack centrioles. In mouse oocytes, spindle microtubules are nucleated from multiple acentriolar MTOCs that are sorted and clustered prior to completion of spindle assembly in an "inside-out" mechanism, ending with establishment of the poles. We used HSET (kinesin-14) as a tool to shift meiotic spindle assembly toward a mitotic "outside-in" mode and analyzed the consequences on the fidelity of the division. We show that HSET levels must be tightly gated in meiosis I and that even slight overexpression of HSET forces spindle morphogenesis to become more mitotic-like: rapid spindle bipolarization and pole assembly coupled with focused poles. The unusual length of meiosis I is not sufficient to correct these early spindle morphogenesis defects, resulting in severe chromosome alignment abnormalities. Thus, the unique "inside-out" mechanism of meiotic spindle assembly is essential to prevent chromosomal misalignment and production of aneuploidy gametes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.