ArticlePloS one2018
Selection of housekeeping genes and demonstration of RNAi in cotton leafhopper, Amrasca biguttula biguttula (Ishida).
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 57 citations in OpenAlex.
- A model system test of trophic transmission of RNA interference effects using the milkweed bug Oncopeltus fasciatus (Hemiptera).Scientific reports · 2026Article
- RNAi-Based Approach as a Promising Tool for Management of Leafhopper (Amrasca biguttula biguttula) in Cotton.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Short-Term Evolutionary Features and Circadian Clock-Modulated Gene Expression Analysis ofInsects · 2025Article
- Molecular approaches for the management of papaya ringspot virus infecting papaya: a comprehensive review.Molecular biology reports · 2024Review
- Stable internal reference genes for quantitative RT-PCR analyses in Rhipicephalus microplus during embryogenesis.Ticks and tick-borne diseases · 2023Article
- Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted BollwormInternational journal of molecular sciences · 2023Article
- Molecular mechanisms underlying host-induced gene silencing.The Plant cell · 2022Review
- Assessing Macro Disease Index of Wheat Stripe Rust Based on Segformer with Complex Background in the Field.Sensors (Basel, Switzerland) · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
- Identification and Validation of Common Reference Genes for Normalization of Esophageal Squamous Cell Carcinoma Gene Expression Profiles.BioMed research international · 2022Article
- Optimizing Efficient RNAi-Mediated Control of Hemipteran Pests (Psyllids, Leafhoppers, Whitefly): Modified Pyrimidines in dsRNA Triggers.Plants (Basel, Switzerland) · 2021Article
- Transcriptome analysis unravels RNAi pathways genes and putative expansion of CYP450 gene family in cotton leafhopper Amrasca biguttula (Ishida).Molecular biology reports · 2021Article
- Reference Gene Selection for Normalizing Gene Expression inFrontiers in physiology · 2021Article
- Effectiveness of orally-delivered double-stranded RNA on gene silencing in the stinkbug Plautia stali.PloS one · 2021Article
- Next Generation dsRNA-Based Insect Control: Success So Far and Challenges.Frontiers in plant science · 2021Review
- Development of a universal endogenous qPCR control for eukaryotic DNA samples.Plant methods · 2020Article
- Enhancing RNAi Efficiency to Decipher the Functional Response of Potential Genes inFrontiers in physiology · 2020Article
- Occurrence prediction of pests and diseases in cotton on the basis of weather factors by long short term memory network.BMC bioinformatics · 2019Article
- mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression.Molecular biology reports · 2019Article
- Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae).Scientific reports · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Amrasca biguttula biguttula (Ishida) commonly known as cotton leafhopper is a severe pest of cotton and okra. Not much is known on this insect at molecular level due to lack of genomic and transcriptomic data. To prepare for functional genomic studies in this insect, we evaluated 15 common housekeeping genes (Tub, B-Tub, EF alpha, GADPH, UbiCF, RP13, Ubiq, G3PD, VATPase, Actin, 18s, 28s, TATA, ETF, SOD and Cytolytic actin) during different developmental stages and under starvation stress. We selected early (1st and 2nd), late (3rd and 4th) stage nymphs and adults for identification of stable housekeeping genes using geNorm, NormFinder, BestKeeper and RefFinder software. Based on the different algorithms, RP13 and VATPase are identified as the most suitable reference genes for quantification of gene expression by reverse transcriptase quantitative PCR (RT-qPCR). Based on RefFinder which comprehended the results of three algorithms, RP13 in adults, Tubulin (Tub) in late nymphs, 28S in early nymph and UbiCF under starvation stress were identified as the most stable genes. We also developed methods for feeding double-stranded RNA (dsRNA) incorporated in the diet. Feeding dsRNA targeting Snf7, IAP, AQP1, and VATPase caused 56.17-77.12% knockdown of targeted genes compared to control and 16 to 48% mortality of treated insects when compared to control.
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