ArticleInternational journal of molecular sciences2018
Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality.
Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives.International journal of molecular sciences · 2018Pooled it
- Acellular lung matrix scaffold coated by fibronectin enhances hepatic differentiation of adipose-derived mesenchymal stem cells.Anatomy & cell biology · 2026Article
- Article
- Modular 3D culture platform combining methylcellulose, testicular ECM, and Sertoli cells forIranian journal of basic medical sciences · 2026Article
- Testicular organoid models in male reproductive toxicology: advances, applications, and future perspectives.Frontiers in physiology · 2026Article
- Article
- Building a human pluripotent stem cell-based gonadal niche: improving in vitro systems with in vivo insights.Human reproduction update · 2025Review
- Harnessing TGF-β signaling to improve testicular organoid development from dissociated testicular cells.Stem cell research & therapy · 2025Article
- Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations.Exploration (Beijing, China) · 2025Review
- In Vitro Spermatogenesis on Human Decellularized Testicular Matrix Plates Following Exosome Treatment in a Dynamic Culture System.Stem cell reviews and reports · 2025Article
- Cryopreservation and culture strategies for testicular tissue and cells in small and large animals.Frontiers in veterinary science · 2025Review
- The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice.Journal of assisted reproduction and genetics · 2024Article
- In vitro spermatogenesis in artificial testis: current knowledge and clinical implications for male infertility.Cell and tissue research · 2023Review
- The Decellularized Calf Testis: Introducing Suitable Scaffolds for Spermatogenesis Studies.International journal of fertility & sterility · 2023Article
- Optimizing Immature Testicular Tissue and Cell Transplantation Results: Comparing Transplantation Sites and Scaffolds.International journal of fertility & sterility · 2023Review
- New Solutions for Old Problems: How Reproductive Tissue Engineering Has Been Revolutionizing Reproductive Medicine.Annals of biomedical engineering · 2023Review
- Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis.Stem cell research & therapy · 2023Review
- In Vitro and In Vivo Models for Drug Transport Across the Blood-Testis Barrier.Drug metabolism and disposition: the biological fate of chemicals · 2023Review
- The Renaissance of Male Infertility Management in the Golden Age of Andrology.The world journal of men's health · 2023Review
- Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?Frontiers in endocrinology · 2023Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryopreservation of immature testicular tissue before chemo/radiotherapy is the only option to preserve fertility of cancer-affected prepubertal boys. To avoid reintroduction of malignant cells, development of a transplantable scaffold by decellularization of pig immature testicular tissue (ITT) able to support decontaminated testicular cells could be an option for fertility restoration in these patients. We, therefore, compared decellularization protocols to produce a cytocompatible scaffold. Fragments of ITT from 15 piglets were decellularized using three protocols: sodium dodecyl sulfate (SDS)-Triton (ST), Triton-SDS-Triton (TST) and trypsin 0.05%/ethylenediaminetetraacetic acid (EDTA) 0.02%-Triton (TET) with varying detergent concentrations. All protocols were able to lower DNA levels. Collagen retention was demonstrated in all groups except ST 1%, and a significant decrease in glycosaminoglycans was observed in the TST 1% and TET 1% groups. When Sertoli cells (SCs) were cultured with decellularized tissue, no signs of cytotoxicity were detected. A higher SC proliferation rate and greater stem cell factor secretion were observed than with SCs cultured without scaffold. ST 0.01% and TET 3% conditions offered the best compromise in terms of DNA elimination and extracellular matrix (ECM) preservation, while ensuring good attachment, proliferation and functionality of human SCs. This study demonstrates the potential of using decellularized pig ITT for human testicular tissue engineering purposes.
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