ArticleFolia microbiologica2018
Overexpression of spoT gene in coccoid forms of clinical Helicobacter pylori isolates.
Article in Folia microbiologica, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Viable but non-cultivable state in oral microbiota: a critical review of an underexplored microbial survival strategy.Frontiers in cellular and infection microbiology · 2025Review
- Study of the relationships among known virulence genes, coccoid transformation and cytotoxicity ofVirulence · 2024Article
- Article
- Review
- Research progress in theZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2021Article
- In Silico Screening and In Vitro Assessment of Natural Products with Anti-Virulence Activity againstMolecules (Basel, Switzerland) · 2021Article
- Biofilm Formation as a Complex Result of Virulence and Adaptive Responses ofPathogens (Basel, Switzerland) · 2020Review
- Review on Stress Tolerance inFrontiers in cellular and infection microbiology · 2020Review
- Application of 16S rRNA gene sequencing inPeerJ · 2020Article
- Clinical and laboratory importance of detectingPrzeglad gastroenterologiczny · 2020Review
- Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference toFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Helicobacter pylori (H. pylori) can convert to coccoid form in unfavorable conditions or as a result of antibiotic treatment. In order to adapt to harsh environments, H. pylori requires a stringent response which, encoded by the spoT gene, has a bifunctional enzyme possessing both (p)ppGpp synthetic and degrading activity. Our goal in this study was to compare spoT gene expression in spiral and induced coccoid forms of H. pylori with use of amoxicillin. First, clinical isolate coccoid forms were induced with amoxicillin; then, the viability test was analyzed by flow cytometer. After RNA extraction, cDNA synthesis and designing a specific primer for spoT gene, evaluation of the desired gene expression in both forms were studied. Bacterial isolates exposed to amoxicillin at MIC and 1/2 MIC induced morphological conversion better and faster than other MIC concentration. The expression of spoT gene was significantly downregulated in spiral forms of H. pylori, while the gene expression was upregulated and + 30.3-fold changes was seen in coccoid forms of bacterium. To summarize, spoT gene is one of the key factors for antibiotic resistance and its enhanced expression in coccoid form can be a valuable diagnostic marker for recognition of H. pylori during morphological conversion.
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Registered trials
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