ArticleLab on a chip2018
A fluorescent microbead-based microfluidic immunoassay chip for immune cell cytokine secretion quantification.
Article in Lab on a chip, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed.
- Review
- Integration of secreted signaling molecule sensing on cell monitoring platforms: a critical review.Analytical and bioanalytical chemistry · 2024Review
- An early HMGB1 rise 12 hours before creatinine predicts acute kidney injury and multiple organ failure in a smoke inhalation and burn swine model.Frontiers in immunology · 2024Article
- Quantitative Evaluation of Interleukin-4 by Immunowall Devices Made of Gelatin Methacryloyl Hydrogel.Molecules (Basel, Switzerland) · 2023Article
- A Microfluidic Cell Co-Culture Chip for the Monitoring of Interactions between Macrophages and Fibroblasts.Biosensors · 2022Article
- A Microfluidic Platform Revealing Interactions between Leukocytes and Cancer Cells on Topographic Micropatterns.Biosensors · 2022Article
- Microfluidic system for immune cell activation and inflammatory cytokine profiling: Application to screening of dietary supplements for anti-inflammatory properties.Biomicrofluidics · 2022Article
- Cytokines: From Clinical Significance to Quantification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Intestinal Stem Cell-on-Chip to Study Human Host-Microbiota Interaction.Frontiers in immunology · 2021Review
- Microfluidic Viscometer Using a Suspending Micromembrane for Measurement of Biosamples.Micromachines · 2020Article
- Article
- Quantification of Protein Secretion from Circulating Tumor Cells in Microfluidic Chambers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Multiplexed Immunosensors and Immunoarrays.Analytical chemistry · 2020Review
- Microphysiological sensing platform for an in-situ detection of tissue-secreted cytokines.Biosensors & bioelectronics: X · 2019Article
- Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-Stem cells international · 2019Article
- Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays.Lab on a chip · 2018Article
- 3D microfluidic in vitro model and bioinformatics integration to study the effects of Spatholobi Caulis tannin in cervical cancer.Scientific reports · 2018Article
- Microfluidic implementation of functional cytometric microbeads for improved multiplexed cytokine quantification.Biomicrofluidics · 2018Article
- Detection of anti-p53 autoantibodies in saliva using microfluidic chips for the rapid screening of oral cancer.RSC advances · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Quantitative and dynamic analyses of immune cell secretory cytokines are essential for precise determination and characterization of the "immune phenotype" of patients for clinical diagnosis and treatment of immune-related diseases. Although multiple methods including the enzyme-linked immunosorbent assay (ELISA) have been applied for cytokine detection, such measurements remain very challenging in real-time, high-throughput, and high-sensitivity immune cell analysis. In this paper, we report a highly integrated microfluidic device that allows for on-chip isolation, culture, and stimulation, as well as sensitive and dynamic cytokine profiling of immune cells. Such a microfluidic sensing chip is integrated with cytometric fluorescent microbeads for real-time and multiplexed monitoring of immune cell cytokine secretion dynamics, consuming a relatively small extracted sample volume (160 nl) without interrupting the immune cell culture. Furthermore, it is integrated with a Taylor dispersion-based mixing unit in each detection chamber that shortens the immunoassay period down to less than 30 minutes. We demonstrate the profiling of multiple pro-inflammatory cytokine secretions (e.g. interleukin-6, interleukin-8, and tumor necrosis factors) of human peripheral blood mononuclear cells (PBMCs) with a sensitivity of 20 pg ml
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.