ArticlePloS one2018
Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
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- First direct evidence for direct cell-membrane penetrations of polycationic homopoly(amino acid)s produced by bacteria.Communications biology · 2022Article
- Genome Characterization and Probiotic Potential ofMicroorganisms · 2022Article
- A Multidisciplinary Approach to Unraveling the Natural Product Biosynthetic Potential of aMicroorganisms · 2021Article
- Alkaloids in Contemporary Drug Discovery to Meet Global Disease Needs.Molecules (Basel, Switzerland) · 2021Review
- A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster.The Journal of antibiotics · 2021Article
- Biosynthesis Gene Cluster and Oxazole Ring Formation Enzyme for Inthomycins inApplied and environmental microbiology · 2020Article
- Mini review: Genome mining approaches for the identification of secondary metabolite biosynthetic gene clusters inComputational and structural biotechnology journal · 2020Review
- Chemical Elicitors of Antibiotic Biosynthesis in Actinomycetes.Microorganisms · 2018Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phthoxazolin A, an oxazole-containing polyketide, has a broad spectrum of anti-oomycete activity and herbicidal activity. We recently identified phthoxazolin A as a cryptic metabolite of Streptomyces avermitilis that produces the important anthelmintic agent avermectin. Even though genome data of S. avermitilis is publicly available, no plausible biosynthetic gene cluster for phthoxazolin A is apparent in the sequence data. Here, we identified and characterized the phthoxazolin A (ptx) biosynthetic gene cluster through genome sequencing, comparative genomic analysis, and gene disruption. Sequence analysis uncovered that the putative ptx biosynthetic genes are laid on an extra genomic region that is not found in the public database, and 8 open reading frames in the extra genomic region could be assigned roles in the biosynthesis of the oxazole ring, triene polyketide and carbamoyl moieties. Disruption of the ptxA gene encoding a discrete acyltransferase resulted in a complete loss of phthoxazolin A production, confirming that the trans-AT type I PKS system is responsible for the phthoxazolin A biosynthesis. Based on the predicted functional domains in the ptx assembly line, we propose the biosynthetic pathway of phthoxazolin A.
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