Evidence map›Paper›PMID 29324290›Full record

ArticleVirology2018

Ross River virus envelope glycans contribute to disease through activation of the host complement system.

Bronwyn M Gunn, Jennifer E Jones, Reed S Shabman, Alan C Whitmore, Sanjay Sarkar, Lance K Blevins, Thomas E Morrison, Mark T Heise

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. In the Crosshairs: RNA Viruses OR Complement?Frontiers in immunology · 2020
    Review
  8. Immunopathogenesis of alphaviruses.Advances in virus research · 2020
    Article
  9. Review
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Bronwyn M GunnDept. of Microbiology and Immunology, University of North Carolina at Chapel Hill, 160 Dental Circle, 9024 Burnett Womack, Chapel Hill, NC 27599, USA. Electronic address: bgunn@mgh.harvard.edu.
Jennifer E JonesDept. of Microbiology and Immunology, University of North Carolina at Chapel Hill, 160 Dental Circle, 9024 Burnett Womack, Chapel Hill, NC 27599, USA. Electronic address: jejxyz@email.unc.edu.
Reed S ShabmanDept. of Microbiology and Immunology, University of North Carolina at Chapel Hill, 160 Dental Circle, 9024 Burnett Womack, Chapel Hill, NC 27599, USA.
Alan C WhitmoreDept. of Genetics, University of North Carolina at Chapel Hill, 160 Dental Circle, 9024 Burnett Womack, Chapel Hill, NC 27599, USA. Electronic address: alan_whitmore@med.unc.edu.
Sanjay SarkarDept. of Genetics, University of North Carolina at Chapel Hill, 160 Dental Circle, 9024 Burnett Womack, Chapel Hill, NC 27599, USA. Electronic address: sanjay_sarkar@med.unc.edu.
Lance K BlevinsDept. of Genetics, University of North Carolina at Chapel Hill, 160 Dental Circle, 9024 Burnett Womack, Chapel Hill, NC 27599, USA.
Thomas E MorrisonDept. of Microbiology, University of Colorado School of Medicine, 12800 E. 19th Ave., RC1N 9119, Mail Stop 8333, Aurora, CO 80045, USA. Electronic address: thomas.morrison@ucdenver.edu.
Mark T HeiseDept. of Microbiology and Immunology, University of North Carolina at Chapel Hill, 160 Dental Circle, 9024 Burnett Womack, Chapel Hill, NC 27599, USA; Dept. of Genetics, University of North Carolina at Chapel Hill, 160 Dental Circle, 9024 Burnett Womack, Chapel Hill, NC 27599, USA. Electronic address: mark_heisem@med.unc.edu.
University of North Carolina at Chapel Hill · USUniversity of Colorado Denver · US

Funding

Yessinia autotransporters (Yaps): Structure, function, and host responseU54AI057157 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI SEMPOWSKI, GREGORY D · 2003 to 2013
$91.4M
Unlocking Zika Virus Immune Control and Pathogenesis with the Collaborative CrossU19AI100625 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI HEISE, MARK T · 2012 to 2021
$36.6M
Translational CoreU19AI109761 · NIAID · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI BARIC, RALPH S · 2014 to 2018
$32.6M
Molecular Biology of Viral Diseases Predoctoral Training GrantT32AI007419 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Mark T Heise, CARY A MOODY · 1993 to 2026
$4.7M
TOGAVIRUS TROPISM FOR BONES, JOINTS, AND CNSR01AR047190 · NIAMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI HEISE, MARK T · 2000 to 2010
$2.6M
NIAID NIH HHS T32 AI007419NIAID NIH HHS U19 AI100625NIAID NIH HHS U19 AI109761NIAID NIH HHS U54 AI057157NIAMS NIH HHS R01 AR047190
6 · The paper itself

Abstract

Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis. As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL. Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans. Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle. Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues. These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis.

Indexed as

Alphavirus InfectionsAnimalsComplement ActivationComplement System ProteinsDisease Models, AnimalHumansMannose-Binding LectinMice, Inbred C57BLPolysaccharidesRoss River virusViral Envelope ProteinsComplement System ProteinsMannose-Binding LectinPolysaccharidesViral Envelope ProteinsAlphavirusComplementMannose-binding lectinN-linked glycanRoss River virus

Identifiers

PMID29324290
PMCPMC7119116
OpenAlexW2782732856

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.