ArticleAnalytical chemistry2018
A pH-Sensing Optode for Mapping Spatiotemporal Gradients in 3D Paper-Based Cell Cultures.
Article in Analytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Opportunities and challenges for analytical chemists: organ-on-chip devices as new approach methods (NAMs) for identifying potential toxins.The Analyst · 2026Review
- Transforming Cancer Diagnosis and Therapy Through Fluorescent Hydrogels: A Review.Advanced healthcare materials · 2026Review
- Innovations in ion-selective optodes: a comprehensive exploration of modern designs and nanomaterial integration.Frontiers in bioengineering and biotechnology · 2024Review
- Knockout cancer by nano-delivered immunotherapy using perfusion-aided scaffold-based tumor-on-a-chip.Nanotheranostics · 2024Review
- Triple-Barrel Ultramicroelectrodes for Multipurpose, Submilliliter Electroanalysis.Analytical chemistry · 2023Article
- Nanocomposite Hydrogels as Functional Extracellular Matrices.Gels (Basel, Switzerland) · 2023Review
- Cellular Invasion Assay for the Real-Time Tracking of Individual Cells in Spheroid or Tumor-like Mimics.Analytical chemistry · 2023Article
- Critical review of polymer and hydrogel deposition methods for optical and electrochemical bioanalytical sensors correlated to the sensor's applicability in real samples.Analytical and bioanalytical chemistry · 2023Review
- Developments on the Smart Hydrogel-Based Drug Delivery System for Oral Tumor Therapy.Gels (Basel, Switzerland) · 2022Review
- Biosensors to Monitor Cell Activity in 3D Hydrogel-Based Tissue Models.Sensors (Basel, Switzerland) · 2022Review
- Analysis of the Relative Humidity Response of Hydrophilic Polymers for Optical Fiber Sensing.Polymers · 2022Article
- Spatially resolved quantification of drug metabolism and efficacy in 3D paper-based tumor mimics.Analytica chimica acta · 2021Article
- Highly Sensitive Fluorescent pH Microsensors Based on the Ratiometric Dye Pyranine Immobilized on Silica Microparticles.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Electrochemical Sensing in 3D Cell Culture Models: New Tools for Developing Better Cancer Diagnostics and Treatments.Cancers · 2021Review
- Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications.Chemical reviews · 2020Article
- Real-time monitoring of extracellular pH using a pH-potentiometric sensing SECM dual-microelectrode.Analytical and bioanalytical chemistry · 2020Article
- Review
- Biomedical Application of Functional Materials in Organ-on-a-Chip.Frontiers in bioengineering and biotechnology · 2020Review
- Luminescent Nanosensors for Ratiometric Monitoring of Three-Dimensional Oxygen Gradients in Laboratory and Clinical Pseudomonas aeruginosa Biofilms.Applied and environmental microbiology · 2019Article
- Review of 3D Cell Culture with Analysis in Microfluidic Systems.Analytical methods : advancing methods and applications · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Paper-based cultures are an emerging platform for preparing 3D tissue-like structures. Chemical gradients can be imposed upon these cultures, generating microenvironments similar to those found in poorly vascularized tumors. There is increasing evidence that the tumor microenvironment is responsible for promoting drug resistance and increased invasiveness. Acidosis, or the acidification of the extracellular space, is particularly important in promoting these aggressive cancer phenotypes. To better understand how cells respond to acidosis there is a need for 3D culture platforms that not only model relevant disease states but also contain sensors capable of quantifying small molecules in the extracellular environment. In this work, we describe pH-sensing optodes that are capable of generating high spatial and temporal resolution maps of pH gradients in paper-based cultures. This sensor was fabricated by suspending microparticles containing pH-sensitive (fluorescein) and pH-insensitive (diphenylanthracene) dyes in a polyurethane hydrogel, which was then coated onto a transparent film. The pH-sensing films have a fast response time, are reversible, stable in long-term culture environments, have minimal photobleaching, and are not cytotoxic. These films have a pK
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.