ArticleScientific reports2018
Expression of cry2Ah1 and two domain II mutants in transgenic tobacco confers high resistance to susceptible and Cry1Ac-resistant cotton bollworm.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- Synergistic Insecticidal Activity AgainstInternational journal of molecular sciences · 2025Article
- Impact of codon optimization onFrontiers in plant science · 2025Article
- In maize, co-expression of GAT and GR79-EPSPS provides high glyphosate resistance, along with low glyphosate residues.aBIOTECH · 2023Article
- The level of Cry1Ac endotoxin and its efficacy againstGM crops & food · 2021Article
- Molecular and Toxicity Analyses of White Granulated Sugar and Other Processing Products Derived From Transgenic Sugarcane.Frontiers in plant science · 2020Article
- Generation of Cry11 Variants ofEvolutionary bioinformatics online · 2020Article
- Resistance status of Helicoverpa armigera against Bt cotton in Pakistan.Transgenic research · 2019Article
- Applications of Protein Engineering and Directed Evolution in Plant Research.Plant physiology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To improve the novel Bacillus thuringiensis insecticidal gene cry2Ah1 toxicity, two mutants cry2Ah1-vp (V354VP) and cry2Ah1-sp (V354SP) were performed. SWISS-MODEL analysis showed two mutants had a longer loop located between β-4 and β-5 of domain II, resulting in higher binding affinity with brush border membrane vesicles (BBMV) of Helicoverpa armigera comparing with Cry2Ah1. The cry2Ah1, cry2Ah1-vp, and cry2Ah1-sp were optimized codon usage according to plant codon bias, and named mcry2Ah1, mcry2Ah1-vp, and mcry2Ah1-sp. They were transformed into tobacco via Agrobacterium-mediated transformation and a total of 4, 8, and 24 transgenic tobacco plants were obtained, respectively. The molecular detection showed the exogenous gene was integrated into tobacco genome, and successfully expressed at the transcript and translation levels. Cry2Ah1 protein in transgenic tobacco plants varied from 4.41 to 40.28 μg g
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.