ArticleScientific reports2018
Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- NOP56 is essential for mammalian generation and maintenance of multiple central nervous systems, associated with SCA36 pathology.Acta neuropathologica communications · 2026Article
- IRF2 deficiency disrupts pyroptosis, NK cell interferon-γ production and resistance to Francisella.EMBO reports · 2026Article
- Establishment of CRISPR-Cas9-Mediated Gene Editing in the Swimming CrabMolecules (Basel, Switzerland) · 2026Article
- Intravitreal adenine base editing of RS1 improves vision in a preclinical mouse model of retinoschisis.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Quantitative measures of clock protein dynamics in the mouse suprachiasmatic nucleus extends the circadian time-keeping model.The EMBO journal · 2025Article
- Deletion of an evolutionarily conserved TAD boundary impacts spermatogenesis in mice†.Biology of reproduction · 2025Article
- Aflatoxin BEnvironmental and molecular mutagenesis · 2025Article
- Artificial Insemination as a Possible Convenient Tool to Acquire Genome-Edited Mice via In Vivo Fertilization with Engineered Sperm.Biotech (Basel (Switzerland)) · 2024Review
- Comparison Between Electroporation at Different Voltage Levels and Microinjection to Generate Porcine Embryos with Multiple Xenoantigen Knock-Outs.International journal of molecular sciences · 2024Article
- Article
- Perspectives in Genome-Editing Techniques for Livestock.Animals : an open access journal from MDPI · 2023Review
- Nanoparticle-mediated genome editing in single-cell embryos via peptide nucleic acids.Bioengineering & translational medicine · 2023Article
- Gait Abnormalities and Aberrant D2 Receptor Expression and Signaling in Mice Carrying the Human Pathogenic MutationMolecular pharmacology · 2023Article
- Efficient DNA knock-in using AAV-mediated delivery with 2-cell embryo CRISPR-Cas9 electroporation.Frontiers in genome editing · 2023Article
- Electroporation-Mediated CRISPR/Cas9 Genome Editing in Rat Zygotes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- CRISMERE Chromosome Engineering in Mouse and Rat.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Pigs with anFrontiers in cell and developmental biology · 2023Article
- Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes.Journal of visualized experiments : JoVE · 2022Article
- Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate.The Journal of reproduction and development · 2022Article
- JUN Regulation of Injury-Induced Enhancers in Schwann Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this report, we present an improved protocol for CRISPR/Cas9 genome editing in mice. The procedure consists in the electroporation of intact mouse zygotes with ribonucleoprotein complexes prepared in vitro from recombinant Cas9 nuclease and synthetic dual guide RNA. This simple cloning-free method proves to be extremely efficient for the generation of indels and small deletions by non-homologous end joining, and for the generation of specific point mutations by homology-directed repair. The procedure, which avoids DNA construction, in vitro transcription and oocyte microinjection, greatly simplifies genome editing in mice.
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