ArticleScientific reports2018
Reference gene identification and validation for quantitative real-time PCR studies in developing Xenopus laevis.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
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- Developmental Patterns of Hepatic Peroxisome Proliferator-Activated Receptor (PPAR) Expression in Xenopus laevis and Response to Pharmaceutical Agonists During Metamorphic Climax.Journal of experimental zoology. Part A, Ecological and integrative physiology · 2025Article
- Reference genes for Eucalyptus spp. under Beauveria bassiana inoculation and subsequently infestation by the galling wasp Leptocybe invasa.Scientific reports · 2024Article
- Gene expression analysis of the Tao kinase family of Ste20p-like map kinase kinase kinases during early embryonic development in Xenopus laevis.Gene expression patterns : GEP · 2023Article
- Identification and validation of the reference genes in the echiuran worm Urechis unicinctus based on transcriptome data.BMC genomics · 2023Article
- Insight into the Molecule Impact of Critical-Sized UHMWPE-ALN Wear Particles on Cells by the Alginate-Encapsulated Cell Reactor.International journal of molecular sciences · 2023Article
- Understanding the Role of ATP Release through Connexins Hemichannels during Neurulation.International journal of molecular sciences · 2023Article
- Systematic identification of reference genes for qRT-PCR ofFrontiers in plant science · 2023Article
- Alcohol induces neural tube defects by reducing retinoic acid signaling and promoting neural plate expansion.Frontiers in cell and developmental biology · 2023Article
- Bisphenol A Interferes with Redox Balance and the Nrf2 Signaling Pathway inAnimals : an open access journal from MDPI · 2022Article
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- Identification of Reference Genes for Reverse Transcription-Quantitative PCR Analysis of Ginger Under Abiotic Stress and for Postharvest Biology Studies.Frontiers in plant science · 2022Article
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- Conserved role of the urotensin II receptor 4 signalling pathway to control body straightness in a tetrapod.Open biology · 2021Article
- Optimized design of antisense oligomers for targeted rRNA depletion.Nucleic acids research · 2021Article
- Human Health and Ocean Pollution.Annals of global health · 2020Review
- Analysis of expression levels of markers associated with tumor proliferation and angiogenesis in familial adenomatous polyposis.Molecular genetics & genomic medicine · 2020Article
- Identification and evaluation of reference genes for quantitative real-time PCR analysis in Passiflora edulis under stem rot condition.Molecular biology reports · 2020Article
- Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects.Frontiers in pharmacology · 2020Article
- Sensitive and quantitative probing of pseudouridine modification in mRNA and long noncoding RNA.RNA (New York, N.Y.) · 2019Article
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5 authors.
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Abstract
Reference genes are essential for gene expression analysis when using real-time quantitative PCR (RT-qPCR). Xenopus laevis is a popular amphibian model for studying vertebrate embryogenesis and development. Further, X. laevis is ideal for studying thyroid signaling due to its thyroid dependent metamorphosis, a stage comparable to birth in humans. When using PCR based studies, a primary concern is the choice of reference genes. Commonly used references are eef1a1, odc1, rpl8, and actnB, although there is a lack of ad hoc reference genes for X. laevis. Here, we used previously published RNA-seq data on different X. laevis stages and identified the top 14 candidate genes with respect to their expression levels as a function of developmental stage and degree of variation. We further evaluated the stability of these and other candidate genes using RT-qPCR on various stages including the unfertilised eggs, whole embryos during early development and brains during late development. We used four different statistical software packages: deltaCT, geNorm, NormFinder and BestKeeper. We report optimized reference gene pair combinations for studying development (early whole embryos), brains at later stages (metamorphosis and adult), and thyroid signalling. These reference gene pairs are suitable for studying different aspects of X. laevis development and organogenesis.
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