ArticleInternational journal of organ transplantation medicine2017
Osteoblast Differentiation on Collagen Scaffold with Immobilized Alkaline Phosphatase.
Article in International journal of organ transplantation medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 49 citations in OpenAlex.
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- Osteogenic Potential of Osteolforte: Gene and Protein-Level Evaluation in Human Bone Marrow Stromal Cells.Current issues in molecular biology · 2025Article
- In vitro study on the osteoimmunological potential of magnesium implants (WE43MEO).Biomedical engineering online · 2025Article
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- Layered Double Hydroxides: Recent Progress and Promising Perspectives Toward Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Combination adipose-derived mesenchymal stem cells-demineralized dentin matrix increase bone marker expression in periodontitis rats.The Saudi dental journal · 2023Article
- Three-Dimensional Printing of Polycaprolactone/Nano-Hydroxyapatite Composite Scaffolds with a Pore Size of 300/500 µm is Histocompatible and Promotes Osteogenesis Using Rabbit Cortical Bone Marrow Stem Cells.Annals of transplantation · 2023Article
- Osteocyte-Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model.JBMR plus · 2023Article
- A Novel Porous Butyryl Chitin-Animal Derived Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair.International journal of molecular sciences · 2023Article
- Article
- A comparison of human dental pulp stem cell activity cultured on sandblasted titanium discs decontaminated with Er:YAG laser and air-powder abrasion: an in vitro study.Lasers in medical science · 2022Article
- Influence of the Components and Orientation of Hydroxyapatite Fibrous Substrates on Osteoblast Behavior.Journal of functional biomaterials · 2022Article
- Osteogenic differentiation of adipose-derived stem cells on dihydroartemisinin electrospun nanofibers.Journal of biological engineering · 2022Article
- A comparative assessment of collagen type 1 from silver carp (fresh water) and milk shark(marine) fish waste.3 Biotech · 2022Article
- Effect of Urolithin A on Bone Repair in Mice with Bone Defects.Tissue engineering and regenerative medicine · 2022Article
- Hollow pollen grains as scaffolding building blocks in bone tissue engineering.BioImpacts : BI · 2022Article
- Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn tissue engineering, scaffold characteristics play an important role in the biological interactions between cells and the scaffold. Cell adhesion, proliferation, and activation depend on material properties used for the fabrication of scaffolds.
objectiveIn the present investigation, we used collagen with proper characteristics including mechanically stability, biodegradability and low antigenicity. Optimization of the scaffold was done by immobilization of alkaline phosphatase on the collagen surface via cross-linking method, because this enzyme is one of the most important markers of osteoblast, which increases inorganic phosphate concentration and promote mineralization of bone formation.
methodsAlkaline phosphatase was immobilized on a collagen surface by 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride, as a reagent. Then, rat mesenchymal stem cells were cultured in osteogenic medium in control and treated groups. The osteogenesis-related genes were compared between treatments (differentiated cells with immobilized alkaline phosphatase/collagen scaffold) and control groups (differentiated cells on collagen surface without alkaline phosphatase) on days 3 and 7 by quantitative real-time PCR (QIAGEN software).
resultsSeveral genes, including alkaline phosphatase, collagen type I and osteocalcine associated with calcium binding and mineralization, showed upregulation in expression during the first 3 days, whereas tumor necrosis factor-α, acting as an inhibitor of differentiation, was down-regulated during osteogenesis.
conclusionCollagen scaffold with immobilized alkaline phosphatase can be utilized as a good candidate for enhancing the differentiation of osteoblasts from mesenchymal stem cells.
Indexed as
Identifiers
29321835PMC5756901W2989867950What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.