ArticleScientific reports2018
Distinction of Plasmodium ovale wallikeri and Plasmodium ovale curtisi using quantitative Polymerase Chain Reaction with High Resolution Melting revelation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Comparison of Plasmodium ovale curtisi and Plasmodium ovale wallikeri infections by a meta-analysis approach.Scientific reports · 2021Pooled it
- Performance of High-Resolution Melting Analysis for Molecular Detection and Species Differentiation of Malaria: A Scoping Review.Pathogens (Basel, Switzerland) · 2026Article
- Development of new real-time PCR assays for detection and species differentiation of Plasmodium ovale.PLoS neglected tropical diseases · 2024Article
- A novel sensitive hexaplex high-resolution melt assay for identification of five human Plasmodium species plus internal control.Acta tropica · 2023Article
- Development and Optimization of a Selective Whole-Genome Amplification To Study Plasmodium ovale Spp.Microbiology spectrum · 2022Article
- Evaluation of two commercial kits and two laboratory-developed qPCR assays compared to LAMP for molecular diagnosis of malaria.Malaria journal · 2022Article
- Genetic Diversity and Phylogenetic Relatedness ofMicroorganisms · 2022Article
- An analysis ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- Epidemiological, Physiological and Diagnostic Comparison ofDiagnostics (Basel, Switzerland) · 2021Review
- Plasmodium ovale wallikeri and P. ovale curtisi Infections and Diagnostic Approaches to Imported Malaria, France, 2013-2018.Emerging infectious diseases · 2021Article
- Identification of Reticulocyte Binding Domain ofFrontiers in cellular and infection microbiology · 2021Article
- High resolution melting based method for rapid discriminatory diagnosis of co-infecting Leptomonas seymouri in Leishmania donovani-induced leishmaniasis.Parasitology international · 2020Article
- Evaluation of PCR To Monitor Plasmodium falciparum Treatment Efficacy in a Nonendemicity Setting.Journal of clinical microbiology · 2019Article
- A comparison of two PCR protocols for the differentiation of Plasmodium ovale species and implications for clinical management in travellers returning to Germany: a 10-year cross-sectional study.Malaria journal · 2019Article
- Characterization of Plasmodium ovale spp. imported from Africa to Henan Province, China.Scientific reports · 2019Article
- A new high-resolution melting analysis for the detection and identification of Plasmodium in human and Anopheles vectors of malaria.Scientific reports · 2019Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmodium ovale curtisi (Poc) and Plasmodium ovale wallikeri (Pow) have been described as two distinct species, only distinguishable by molecular methods such as PCR. Because of no well-defined endemic area and a variable clinical presentation as higher thrombocytopenia and nausea associated with Pow infection and asymptomatic forms of the pathology with Poc infection, rapid and specific identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri are needed. The aim of the study was to evaluate a new quantitative real-time PCR coupled with high resolution melting revelation (qPCR-HRM) for identification of both species. Results were compared with a nested-PCR, considered as a gold standard for Pow and Poc distinction. 356 samples including all human Plasmodium species at various parasitaemia were tested. The qPCR-HRM assay allowed Poc and Pow discrimination in 66 samples tested with a limit of detection evaluated at 1 parasite/µL. All these results were concordant with nested-PCR. Cross-reaction was absent with others blood parasites. The qPCR-HRM is a rapid and convenient technique to Poc and Pow distinction.
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