ArticlePloS one2018
Longitudinal micro-CT as an outcome measure of interstitial lung disease in TNF-transgenic mice.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 37 citations in OpenAlex.
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- 3D reconstruction of the bronchial tree of the Gray short-tailed opossum (Monodelphis domestica) in the postnatal period.Journal of anatomy · 2023Article
- Hepatorenal pathologies in TNF-transgenic mouse model of rheumatoid arthritis are alleviated by anti-TNF treatment.Arthritis research & therapy · 2023Article
- Micro-CT-derived ventilation biomarkers for the longitudinal assessment of pathology and response to therapy in a mouse model of lung fibrosis.Scientific reports · 2023Article
- Implementation of automated behavior metrics to evaluate voluntary wheel running effects on inflammatory-erosive arthritis and interstitial lung disease in TNF-Tg mice.Arthritis research & therapy · 2023Article
- Semi-automated micro-computed tomography lung segmentation and analysis in mouse models.MethodsX · 2023Article
- A high-throughput semi-automated bone segmentation workflow for murine hindpaw micro-CT datasets.Bone reports · 2022Article
- Intracellular hydroxyproline imprinting following resolution of bleomycin-induced pulmonary fibrosis.The European respiratory journal · 2022Article
- Computational pathology for musculoskeletal conditions using machine learning: advances, trends, and challenges.Arthritis research & therapy · 2022Review
- Huangqi Guizhi Wuwu Decoction Improves Arthritis and Pathological Damage of Heart and Lung in TNF-Tg Mice.Frontiers in pharmacology · 2022Article
- Altered responses of end-expiratory lung volume and upper airway patency to body posture in diet-induced obese mice.Physiological reports · 2021Article
- Animal models of rheumatoid arthritis-associated interstitial lung disease.Immunity, inflammation and disease · 2021Review
- Simple low dose radiography allows precise lung volume assessment in mice.Scientific reports · 2021Article
- Article
- In-vivo lung fibrosis staging in a bleomycin-mouse model: a new micro-CT guided densitometric approach.Scientific reports · 2020Article
- Tumor Necrosis Factor Induces Obliterative Pulmonary Vascular Disease in a Novel Model of Connective Tissue Disease-Associated Pulmonary Arterial Hypertension.Arthritis & rheumatology (Hoboken, N.J.) · 2020Article
- Longitudinal micro-computed tomography-derived biomarkers quantify non-resolving lung fibrosis in a silicosis mouse model.Scientific reports · 2020Article
- Progression of regional lung strain and heterogeneity in lung injury: assessing the evolution under spontaneous breathing and mechanical ventilation.Annals of intensive care · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
introductionRheumatoid arthritis associated interstitial lung disease (RA-ILD) is a debilitating condition with poor survival prognosis. High resolution computed tomography (CT) is a common clinical tool to diagnose RA-ILD, and is increasingly being adopted in pre-clinical studies. However, murine models recapitulating RA-ILD are lacking, and CT outcomes for inflammatory lung disease have yet to be formally validated. To address this, we validate μCT outcomes for ILD in the tumor necrosis factor transgenic (TNF-Tg) mouse model of RA.
methodsCross sectional μCT was performed on cohorts of male TNF-Tg mice and their WT littermates at 3, 4, 5.5 and 12 months of age (n = 4-6). Lung μCT outcomes measures were determined by segmentation of the μCT datasets to generate Aerated and Tissue volumes. After each scan, lungs were obtained for histopathology and 3 sections stained with hematoxylin and eosin. Automated histomorphometry was performed to quantify the tissue area (nuclei, cytoplasm, and extracellular matrix) and aerated area (white space) within the tissue sections. Spearman's correlation coefficients were used to evaluate the extent of association between μCT imaging and histopathology endpoints.
resultsTNF-Tg mice had significantly greater tissue volume, total lung volume and mean intensity at all timepoints compared to age matched WT littermates. Histomorphometry also demonstrated a significant increase in tissue area at 3, 4, and 5.5 months of age in TNF-Tg mice. Lung tissue volume was correlated with lung tissue area (ρ = 0.81, p<0.0001), and normalize lung aerated volume was correlated with normalized lung air area (ρ = 0.73, p<0.0001).
conclusionsWe have validated in vivo μCT as a quantitative biomarker of ILD in mice. Further, development of longitudinal measures is critical for dissecting pathologic progression of ILD, and μCT is a useful non-invasive method to study lung inflammation in the TNF-Tg mouse model.
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