ArticleNature methods2018
Multicolor quantitative confocal imaging cytometry.
Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 96 citations in OpenAlex.
- Practical AI-based cell extraction and spatial statistics for large 3D bone marrow tissue images.Cell reports methods · 2026Article
- Rapid 3D Immunolabeling and Light Sheet Microscopy for Quantitative Analysis of Intact Tissues.Computational and structural biotechnology journal · 2026Article
- Next generation lineage tracing and its applications to unravel development.NPJ systems biology and applications · 2025Review
- Age- and sex-specific deterioration on bone and osteocyte lacuno-canalicular network in a mouse model of premature aging.Bone research · 2025Article
- Differential regulation of fetal bone marrow and liver hematopoiesis by yolk-sac-derived myeloid cells.Nature communications · 2025Article
- Acute malaria suppresses the B lymphocytic niche in the bone marrow through the alteration of CXCL12-abundant reticular cells.International immunology · 2024Article
- Evolutionary graph theory beyond single mutation dynamics: on how network-structured populations cross fitness landscapes.Genetics · 2024Article
- A theory of evolutionary dynamics on any complex population structure reveals stem cell niche architecture as a spatial suppressor of selection.Nature communications · 2024Article
- IL-1β promotes MPN disease initiation by favoring early clonal expansion of JAK2-mutant hematopoietic stem cells.Blood advances · 2024Article
- Multidimensional Imaging of Breast Cancer.Cold Spring Harbor perspectives in medicine · 2023Review
- B- and T-cell acute lymphoblastic leukemias evade chemotherapy at distinct sites in the bone marrow.Haematologica · 2023Article
- Characterization of Systemic Disease Development and Paw Inflammation in a Susceptible Mouse Model of Mayaro Virus Infection and Validation Using X-ray Synchrotron Microtomography.International journal of molecular sciences · 2023Article
- Intravital Microscopy for Hematopoietic Studies.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D.Nature protocols · 2022Review
- Fast and high-fidelitySTAR protocols · 2022Article
- Open-source personal pipetting robots with live-cell incubation and microscopy compatibility.Nature communications · 2022Article
- 3D imaging for driving cancer discovery.The EMBO journal · 2022Review
- Present Application and Perspectives of Organoid Imaging Technology.Bioengineering (Basel, Switzerland) · 2022Review
- An In Vivo CRISPR Screen Identifies Stepwise Genetic Dependencies of Metastatic Progression.Cancer research · 2022Article
- The extracellular matrix of hematopoietic stem cell niches.Advanced drug delivery reviews · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multicolor 3D quantitative imaging of large tissue volumes is necessary to understand tissue development and organization as well as interactions between distinct cell types in situ. However, tissue imaging remains technically challenging, particularly imaging of bone and marrow. Here, we describe a pipeline to reproducibly generate high-dimensional quantitative data from bone and bone marrow that may be extended to any tissue. We generate thick bone sections from adult mouse femurs with preserved tissue microarchitecture and demonstrate eight-color imaging using confocal microscopy without linear unmixing. We introduce XiT, an open-access software for fast and easy data curation, exploration and quantification of large imaging data sets with single-cell resolution. We describe how XiT can be used to correct for potential artifacts in quantitative 3D imaging, and we use the pipeline to measure the spatial relationship between hematopoietic cells, bone matrix and marrow Schwann cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.