ReviewSynthetic and systems biotechnology2017
Fundamental CRISPR-Cas9 tools and current applications in microbial systems.
Review in Synthetic and systems biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Application of compact CRISPR/Cas nucleases for citrus genome editing.Transgenic research · 2026Article
- Improved vectors for retron-mediated CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2025Article
- Harnessing the evolving CRISPR/Cas9 for precision oncology.Journal of translational medicine · 2024Review
- Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals.International journal of molecular sciences · 2024Review
- CRISPR-Based Therapies: Revolutionizing Drug Development and Precision Medicine.Current gene therapy · 2024Review
- Past, Present, and Future of Genome Modification inMicroorganisms · 2022Review
- Heterologous production of cyanobacterial compounds.Journal of industrial microbiology & biotechnology · 2021Review
- CRISPR/Cas based gene editing: marking a new era in medical science.Molecular biology reports · 2021Review
- Hyperproduction of 3-hydroxypropionate by Halomonas bluephagenesis.Nature communications · 2021Article
- Cytosine Base Editor-Mediated Multiplex Genome Editing to Accelerate Discovery of Novel Antibiotics inFrontiers in microbiology · 2021Article
- Effective Blocking of Microbial Transcriptional Initiation by dCas9-NG-Mediated CRISPR Interference.Journal of microbiology and biotechnology · 2020Article
- Bacterial diversity and functional metagenomics expounding the diversity of xenobiotics, stress, defense and CRISPR gene ontology providing eco-efficiency to Himalayan Hot Springs.Functional & integrative genomics · 2020Article
- CRISPR-Cas9/CRISPRi tools for cell factory construction in E. coli.World journal of microbiology & biotechnology · 2020Review
- Construction of Antisense RNA-mediated Gene Knock-downStrains in the CyanobacteriumBio-protocol · 2020Article
- Transcription Factor Engineering for High-Throughput Strain Evolution and Organic Acid Bioproduction: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- De novo design of programmable inducible promoters.Nucleic acids research · 2019Article
- μLAS technology for DNA isolation coupled to Cas9-assisted targeting for sequencing and assembly of a 30 kb region in plant genome.Nucleic acids research · 2019Article
- Engineering and modification of microbial chassis for systems and synthetic biology.Synthetic and systems biotechnology · 2019Review
- Unintentional Genomic Changes EndowGenes · 2018Article
- CRISPR-Cas9/Cas12a biotechnology and application in bacteria.Synthetic and systems biotechnology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we outline the fundamental CRISPR tools that have been employed for strain optimization. These tools include CRISPR editing, CRISPR interference, CRISPR activation and protein imaging. To further characterize the CRISPR technology, we present current applications of these tools in microbial systems, including model- and non-model industrial microorganisms. Specially, we point out the major challenges of the CRISPR tools when utilized for multiplex genome editing and sophisticated expression regulation. To address these challenges, we came up with strategies that place emphasis on the amelioration of DNA repair efficiency through CRISPR-Cas9-assisted recombineering. Lastly, multiple promising research directions were proposed, mainly focusing on CRISPR-based construction of microbial ecosystems toward high production of desired chemicals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.