ArticleJournal of healthcare engineering2017
Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material.
Article in Journal of healthcare engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- HumanBioactive materials · 2026Article
- Mechanical maturation of human dermal fibroblast-laden microporous annealed particle scaffolds during long-term in vitro culture.Acta biomaterialia · 2025Article
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- Breast cancer brain metastasis: from etiology to state-of-the-art modeling.Journal of biological engineering · 2023Review
- Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature.Journal of personalized medicine · 2022Review
- Spatiotemporal patterns of gene expression around implanted silicon electrode arrays.Journal of neural engineering · 2021Article
- In Vitro Models of the Blood-Brain Barrier.Handbook of experimental pharmacology · 2021Article
- Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration.Bioengineering (Basel, Switzerland) · 2020Review
- Article
- A Parameter Study for 3D-Printing Organized Nanofibrous Collagen Scaffolds Using Direct-Write Electrospinning.Materials (Basel, Switzerland) · 2019Article
- Article
- Recent Progress in Microfluidic Models of the Blood-Brain Barrier.Micromachines · 2019Review
- Establishment of a Human iPSC- and Nanofiber-Based Microphysiological Blood-Brain Barrier System.ACS applied materials & interfaces · 2018Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Disruption of the blood-brain barrier (BBB) is the hallmark of many neurovascular disorders, making it a critically important focus for therapeutic options. However, testing the effects of either drugs or pathological agents is difficult due to the potentially damaging consequences of altering the normal brain microenvironment. Recently, in vitro coculture tissue models have been developed as an alternative to animal testing. Despite low cost, these platforms use synthetic scaffolds which prevent normal barrier architecture, cellular crosstalk, and tissue remodeling. We created a biodegradable electrospun gelatin mat "biopaper" (BP) as a scaffold material for an endothelial/astrocyte coculture model allowing cell-cell contact and crosstalk. To compare the BP and traditional models, we investigated the expression of 27 genes involved in BBB permeability, cellular function, and endothelial junctions at different time points. Gene expression levels demonstrated higher expression of transcripts involved in endothelial junction formation, including TJP2 and CDH5, in the BP model. The traditional model had higher expression of genes associated with extracellular matrix-associated proteins, including SPARC and COL4A1. Overall, the results demonstrate that the BP coculture model is more representative of a healthy BBB state, though both models have advantages that may be useful in disease modeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.