Evidence map›Paper›PMID 29316975›Full record

ArticleBiological research2018

Inducible T-cell co-stimulators regulate the proliferation and invasion of human hepatocellular carcinoma HepG2 cells.

Yaning Wei, Yanan Wang, Aimin Zang, Yanhong Shang, Zizheng Song, Zhiyu Wang, Yangyang Wang, Hua Yang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biological research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Yaning WeiDepartments of Oncology, Affiliated Hospital of Hebei University, Baoding, 071000, China.
Yanan WangDepartment of Pathology, Affiliated Hospital of Hebei University, Baoding, 071000, China.
Aimin ZangDepartments of Oncology, Affiliated Hospital of Hebei University, Baoding, 071000, China.
Yanhong ShangDepartments of Oncology, Affiliated Hospital of Hebei University, Baoding, 071000, China.
Zizheng SongDepartments of Oncology, Affiliated Hospital of Hebei University, Baoding, 071000, China.
Zhiyu WangDepartments of Oncology, Affiliated Hospital of Hebei University, Baoding, 071000, China.
Yangyang WangDepartment of Gastroenterology, Affiliated Hospital of Hebei University, Baoding, 071000, China.
Hua YangDepartments of Oncology, Affiliated Hospital of Hebei University, Baoding, 071000, China. docyh@163.com.
Affiliated Hospital of Hebei University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThis study determined the regulatory effects of inducible T-cell co-stimulators (ICOS) in human hepatocellular carcinoma HepG2 cells using a RNA interference (RNAi) technique.

methodsA RNAi technique was used to knockdown the expression of ICOS. ICOS expression after knockdown was detected as mRNA and protein levels by RT-PCR and Western blot, respectively. A MTT colorimetric assay was used to detect cell proliferation, and the Transwell assay was used to detect cell invasion. Western blot was carried out to detect the level of Bcl-2, AKT, and PI3K protein expression in different groups.

resultsThe proliferation of HepG2 cells were significantly decreased after ICOS siRNA transfection (EG group). Similarly, the results of the Transwell experiment showed that invasion of HepG2 cells in the EG group was clearly reduced compared to the negative control (NC) and blank control groups (CON). Western blot analysis showed that knockdown of ICOS expression reduced the levels of Bcl-2 and AKT, and also significantly up-regulated the level of PI3K phosphorylation (P < 0.01).

conclusionDown-regulating ICOS expression in HepG2 cells suppressed cell proliferation and invasion. The underlying mechanism may be related to the expression of the downstream factor, PI3K/AKT.

Indexed as

Blotting, WesternCarcinoma, HepatocellularCell ProliferationColorimetryDown-RegulationGene Expression Regulation, NeoplasticGene Knockdown TechniquesHep G2 CellsHumansInducible T-Cell Co-Stimulator ProteinLiver NeoplasmsNeoplasm InvasivenessPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-bcl-2Reverse Transcriptase Polymerase Chain ReactionInducible T-Cell Co-Stimulator ProteinPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-bcl-2Inducible T-cell co-stimulator (ICOS)InvasionLiver cancerPI3K/AKTProliferation

Identifiers

PMID29316975
PMCPMC5759367
OpenAlexW2783430375

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.