Evidence map›Paper›PMID 29316958›Full record

ArticleVirology journal2018

Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis.

Emilie Bonin, Stéphane Quéguiner, Cédric Woudstra, Stéphane Gorin, Nicolas Barbier, Timm C Harder, Patrick Fach, Séverine Hervé, Gaëlle Simon

Open access · goldAbstract read
In one paragraph

Article in Virology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Journal of fungi (Basel, Switzerland) · 2021
    Article
  6. Swine influenza A virus subtypes circulating in Brazilian commercial pig herds from 2012 to 2019.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021
    Article
  7. Article
  8. Article
  9. Article
  10. Evaluation of two multiplex RT-PCR assays for detection and subtype differentiation of Brazilian swine influenza viruses.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020
    Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Subtyping of Swine Influenza Viruses Using a High-Throughput Real-Time PCR Platform.Frontiers in cellular and infection microbiology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Emilie BoninANSES, Ploufragan-Plouzané Laboratory, Swine Virology Immunology Unit, National Reference Laboratory for Swine Influenza, Ploufragan, France.
Stéphane QuéguinerANSES, Ploufragan-Plouzané Laboratory, Swine Virology Immunology Unit, National Reference Laboratory for Swine Influenza, Ploufragan, France.
Cédric WoudstraANSES, Laboratory for Food Safety, IdentyPath Platform, Maisons-Alfort, France.
Stéphane GorinANSES, Ploufragan-Plouzané Laboratory, Swine Virology Immunology Unit, National Reference Laboratory for Swine Influenza, Ploufragan, France.
Nicolas BarbierANSES, Ploufragan-Plouzané Laboratory, Swine Virology Immunology Unit, National Reference Laboratory for Swine Influenza, Ploufragan, France.
Timm C HarderInstitute of Diagnostic Virology, Friedrich-Loeffler Institute, Greifswald-Insel Riems, Germany.
Patrick FachANSES, Laboratory for Food Safety, IdentyPath Platform, Maisons-Alfort, France.
Séverine HervéANSES, Ploufragan-Plouzané Laboratory, Swine Virology Immunology Unit, National Reference Laboratory for Swine Influenza, Ploufragan, France.
Gaëlle SimonANSES, Ploufragan-Plouzané Laboratory, Swine Virology Immunology Unit, National Reference Laboratory for Swine Influenza, Ploufragan, France. gaelle.simon@anses.fr.ORCID 0000-0003-1099-5075
National Influenza Center · MNAgence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail · FRFriedrich-Loeffler-Institut · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSwine influenza is a respiratory infection of pigs that may have a significant economic impact in affected herds and pose a threat to the human population since swine influenza A viruses (swIAVs) are zoonotic pathogens. Due to the increasing genetic diversity of swIAVs and because novel reassortants or variants may become enzootic or have zoonotic implications, surveillance is strongly encouraged. Therefore, diagnostic tests and advanced technologies able to identify the circulating strains rapidly are critically important.

resultsSeveral reverse transcription real-time PCR assays (RT-qPCRs) were developed to subtype European swIAVs in clinical samples previously identified as containing IAV genome. The RT-qPCRs aimed to discriminate HA genes of four H1 genetic lineages (H1

conclusionThe RT-qPCRs dedicated to IAV molecular subtyping enabled the identification of swIAVs from the four viral subtypes that are known to be enzootic in European pigs, i.e. H1

Indexed as

AnimalsCell LineDogsEuropeGenes, ViralGenetic VariationGenome, ViralHemagglutinin Glycoproteins, Influenza VirusInfluenza A virusMadin Darby Canine Kidney CellsMolecular TypingOrthomyxoviridae InfectionsReproducibility of ResultsSwineHemagglutinin Glycoproteins, Influenza VirusDiagnosisHemagglutininHigh-throughput real-time RT-PCRInfluenzavirusLightCycler®1536NeuraminidasePigSubtypingSurveillance

Identifiers

PMID29316958
PMCPMC5761149
OpenAlexW2783886200

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.