ReviewCurrent opinion in neurobiology2018
Expansion microscopy: development and neuroscience applications.
Review in Current opinion in neurobiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
- Optical Approaches to Dissect the Structure and Dynamics of the Synapse at Nanoscale Resolution.Advances in neurobiology · 2026Review
- Genetically-encoded markers for confocal visualization of single dense core vesicles.Communications biology · 2025Article
- Extraembryonic mesoderm cells derived from human embryonic stem cells rely on Wnt pathway activation.Cell proliferation · 2025Article
- Accelerated protein retention expansion microscopy using microwave radiation.Cell reports methods · 2024Article
- Super-resolution imaging of the neuronal cytoskeleton.Npj imaging · 2024Review
- Genetically-encoded markers for confocal visualization of single dense core vesicles.Research square · 2024Article
- EDTP enhances and protects the fluorescent signal of GFP in cleared and expanded tissues.Scientific reports · 2024Article
- Accelerated protein retention expansion microscopy using microwave radiation.bioRxiv : the preprint server for biology · 2024Article
- Tau Oligomer-Containing Synapse Elimination by Microglia and Astrocytes in Alzheimer Disease.JAMA neurology · 2023Article
- Heat denaturation enables multicolor X10-STED microscopy.Scientific reports · 2023Article
- Single p197 molecules of the mitochondrial genome segregation system ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Identifying long-range synaptic inputs using genetically encoded labels and volume electron microscopy.Scientific reports · 2022Article
- Recent Microscopy Advances and the Applications to Huntington's Disease Research.Journal of Huntington's disease · 2022Review
- Inhibition of LRRK2 kinase activity promotes anterograde axonal transport and presynaptic targeting of α-synuclein.Acta neuropathologica communications · 2021Article
- Axo-axonic synapses: Diversity in neural circuit function.The Journal of comparative neurology · 2021Review
- Review
- Advanced imaging and labelling methods to decipher brain cell organization and function.Nature reviews. Neuroscience · 2021Review
- Advanced Technologies for Local Neural Circuits in the Cerebral Cortex.Frontiers in neuroanatomy · 2021Article
- Towards a Comprehensive Optical Connectome at Single Synapse ResolutionFrontiers in synaptic neuroscience · 2021Review
- Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Many neuroscience questions center around understanding how the molecules and wiring in neural circuits mechanistically yield behavioral functions, or go awry in disease states. However, mapping the molecules and wiring of neurons across the large scales of neural circuits has posed a great challenge. We recently developed expansion microscopy (ExM), a process in which we physically magnify biological specimens such as brain circuits. We synthesize throughout preserved brain specimens a dense, even mesh of a swellable polymer such as sodium polyacrylate, anchoring key biomolecules such as proteins and nucleic acids to the polymer. After mechanical homogenization of the specimen-polymer composite, we add water, and the polymer swells, pulling biomolecules apart. Due to the larger separation between molecules, ordinary microscopes can then perform nanoscale resolution imaging. We here review the ExM technology as well as applications to the mapping of synapses, cells, and circuits, including deployment in species such as Drosophila, mouse, non-human primate, and human.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.