ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2018
Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichehys nobilis) and silver carp (Hypophthalmichthys molitrix).
Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
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29 citing papers in PubMed, 96 citations in OpenAlex.
- A helix-loop-helix gene orchestrates male fate in common carp.Science advances · 2026Article
- Elucidation of the XX/XY Sex Determination System and Development of a Sex-Linked Molecular Marker in the Freshwater SnailAnimals : an open access journal from MDPI · 2026Article
- Sex-Specific Marker Development and Identification of an XY Sex Determination System in the Tire Track Eel (Animals : an open access journal from MDPI · 2026Article
- Transposable Element-Mediated Cis-Regulation Drives the Evolution of dmrt1 as a Candidate Master Sex-Determining Gene in Black Carp.Molecular biology and evolution · 2025Article
- Role of multi-omics in aquaculture genetics and breeding: current status and future perspective.Science China. Life sciences · 2025Review
- Exploitation and Application of a New Genetic Sex Marker Based on Intron Insertion Variation of erc2 Gene in Oplegnathus punctatus.Marine biotechnology (New York, N.Y.) · 2024Article
- Development of DNA Insertion-specific Markers Based on the Intron Region of Oplegnathus punctatus itih4b for Genetic Sex Identification.Marine biotechnology (New York, N.Y.) · 2024Article
- Genome-Wide Association Studies (GWAS) and Transcriptome Analysis Reveal Male Heterogametic Sex-Determining Regions and Candidate Genes in Northern Snakeheads (International journal of molecular sciences · 2024Article
- Development of DNA Insertion-specific Markers Based on the Intergenic Region of Oplegnathus punctatus Cdkn1/srsf3 for Sex Identification.Marine biotechnology (New York, N.Y.) · 2024Article
- Using a Handful of Transcriptomes to Detect Sex-Linked Markers and Develop Molecular Sexing Assays in a Species with Homomorphic Sex Chromosomes.Genome biology and evolution · 2024Article
- Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus.GigaScience · 2024Article
- Heterogeneous Evolution of Sex Chromosomes in the Torrent Frog GenusInternational journal of molecular sciences · 2022Article
- Article
- Insights into chromosomal evolution and sex determination of Pseudobagrus ussuriensis (Bagridae, Siluriformes) based on a chromosome-level genome.DNA research : an international journal for rapid publication of reports on genes and genomes · 2022Article
- DNA Methylation Difference between Female and Male Ussuri Catfish (Life (Basel, Switzerland) · 2022Article
- Sex-Specific Genomic Region Identification and Molecular Sex Marker Development of Rock Bream (Oplegnathus fasciatus).Marine biotechnology (New York, N.Y.) · 2022Article
- Identification of Sex-Specific Markers Through 2b-RAD Sequencing in the Sea Urchin (Frontiers in genetics · 2021Article
- Updated Genome Assembly of Bighead Carp (Frontiers in genetics · 2021Article
- Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes inFrontiers in endocrinology · 2021Article
- Identification of male heterogametic sex-determining regions on the Atlantic herring Clupea harengus genome.Journal of fish biology · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sex-specific markers are powerful tools for identifying sex-determination system in various animals. Bighead carp (Hypophthalmichehys nobilis) and silver carp (Hypophthalmichthys molitrix) are two of the most important edible fish in Asia, which have a long juvenility period that can lasts for 4-5 years. In this study, we found one sex-specific marker by next-generation sequencing together with bioinformatics analysis in bighead carp. The male-specific markers were used to perform molecular sexing in the progenies of artificial gynogenetic diploids and found all progenies (n = 160) were females. Meanwhile, around 1 : 1 sex ratio was observed in a total of 579 juvenile offspring from three other families. To further extend the male-specific region, we performed genome walking and got a male-specific sequence of 8,661 bp. Five pairs of primers were designed and could be used to efficiently distinguish males from females in bighead carp and silver carp. The development of these male-specific markers and results of their molecular sexing in different populations provide strong evidence for a sex determination system of female homogametry or male heterogametry (XX/XY) in bighead carp and silver carp. To the best of our knowledge, this is the first report of effective sex-specific markers in these two large carp species.
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