ArticleBioengineering & translational medicine2017
Microfluidic co-culture devices to assess penetration of nanoparticles into cancer cell mass.
Article in Bioengineering & translational medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Development and validation of a controlled heating apparatus for long-term MRI of 3D microfluidic tumor models.AIChE journal. American Institute of Chemical Engineers · 2024Article
- Single-cell adhesive profiling in an optofluidic device elucidates CD8Cell reports methods · 2024Article
- Recent advances of nanocrystals in cancer theranostics.Nanoscale advances · 2023Review
- Review
- Patient-Derived In Vitro and In Vivo Models of Cancer.Advances in experimental medicine and biology · 2022Article
- Metastatic disease in head & neck oncology.Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale · 2020Review
- Co-cultured microfluidic model of the airway optimized for microscopy and micro-optical coherence tomography imaging.Biomedical optics express · 2019Article
- The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors.Materials (Basel, Switzerland) · 2019Review
- Editorial: The launch phase ofBioengineering & translational medicine · 2019Article
- Evaluating Nanoparticles in Preclinical Research Using Microfluidic Systems.Micromachines · 2019Review
- A microfluidic model of human brain (μHuB) for assessment of blood brain barrier.Bioengineering & translational medicine · 2019Article
- Modelling cancer in microfluidic human organs-on-chips.Nature reviews. Cancer · 2019Review
- Nanocrystals: A perspective on translational research and clinical studies.Bioengineering & translational medicine · 2019Review
- Design of spherically structured 3D in vitro tumor models -Advances and prospects.Acta biomaterialia · 2018Review
- Detachment of ligands from nanoparticle surface under flow and endothelial cell contact: Assessment using microfluidic devices.Bioengineering & translational medicine · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro and in vivo assessment of safety and efficacy are the essential first steps in developing nanoparticle-based therapeutic systems. However, it is often challenging to use the knowledge gained from in vitro studies to predict the outcome of in vivo studies since the complexity of the in vivo environment, including the existence of flow and a multicellular environment, is often lacking in traditional in vitro models. Here, we describe a microfluidic co-culture model comprising 4T1 breast cancer cells and EA.hy926 endothelial cells under physiological flow conditions and its utilization to assess the penetration of therapeutic nanoparticles from the vascular compartment into a cancerous cell mass. Camptothecin nanocrystals (∼310 nm in length), surface-functionalized with PEG or folic acid, were used as a test nanocarrier. Camptothecin nanocrystals exhibited only superficial penetration into the cancerous cell mass under fluidic conditions, but exhibited cytotoxicity throughout the cancerous cell mass. This likely suggests that superficially penetrated nanocrystals dissolve at the periphery and lead to diffusion of molecular camptothecin deep into the cancerous cell mass. The results indicate the potential of microfluidic co-culture devices to assess nanoparticle-cancerous cell interactions, which are otherwise difficult to study using standard in vitro cultures.
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