ArticleFrontiers in microbiology2017
Construction of Viable Soil Defined Media Using Quantitative Metabolomics Analysis of Soil Metabolites.
Article in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Eco-Corona of Microplastics in Soil Inhibits Their Role as a Contaminant Vector While Enhancing Their Role as a Microorganism Vector.Environmental science & technology · 2026Article
- Synthetic soils for ecological and synthetic biology applications.FEMS microbiology reviews · 2026Review
- Nonliving respiration: Another breath in the soil?Science advances · 2025Article
- Microfluidic droplets with amended culture media cultivate a greater diversity of soil microorganisms.Applied and environmental microbiology · 2025Article
- Soil metabolomics: Deciphering underground metabolic webs in terrestrial ecosystems.Eco-Environment & Health · 2024Review
- Developing stable, simplified, functional consortia fromFrontiers in microbiology · 2024Article
- Green microalgaFrontiers in microbiology · 2024Article
- The core metabolome and root exudation dynamics of three phylogenetically distinct plant species.Nature communications · 2023Article
- Balancing Trade-Offs Imposed by Growth Media and Mass Spectrometry for Bacterial Exometabolomics.Applied and environmental microbiology · 2022Article
- Article
- Nutrient Availability Shifts the Biosynthetic Potential of Soil-Derived Microbial Communities.Current microbiology · 2022Article
- Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies.Frontiers in microbiology · 2022Article
- A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria.Frontiers in microbiology · 2022Article
- Root exudates and rhizosphere soil bacterial relationships ofFrontiers in plant science · 2022Article
- Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions inFrontiers in microbiology · 2021Article
- Nucleoside Metabolism Is Induced in Common Bean During Early Seedling Development.Frontiers in plant science · 2021Article
- Ecophysiological Study ofApplied and environmental microbiology · 2020Article
- Root morphology and exudate availability are shaped by particle size and chemistry inPlant direct · 2020Article
- Local Phenomena Shape Backyard Soil Metabolite Composition.Metabolites · 2020Article
- Evaluation of an untargeted nano-liquid chromatography-mass spectrometry approach to expand coverage of low molecular weight dissolved organic matter in Arctic soil.Scientific reports · 2019Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exometabolomics enables analysis of metabolite utilization of low molecular weight organic substances by soil bacteria. Environmentally-based defined media are needed to examine ecologically relevant patterns of substrate utilization. Here, we describe an approach for the construction of defined media using untargeted characterization of water soluble soil microbial metabolites from a saprolite soil collected from the Oak Ridge Field Research Center (ORFRC). To broadly characterize metabolites, both liquid chromatography mass spectrometry (LC/MS) and gas chromatography mass spectrometry (GC/MS) were used. With this approach, 96 metabolites were identified, including amino acids, amino acid derivatives, sugars, sugar alcohols, mono- and di-carboxylic acids, nucleobases, and nucleosides. From this pool of metabolites, 25 were quantified. Molecular weight cut-off filtration determined the fraction of carbon accounted for by the quantified metabolites and revealed that these soil metabolites have an uneven quantitative distribution (e.g., trehalose accounted for 9.9% of the <1 kDa fraction). This quantitative information was used to formulate two soil defined media (SDM), one containing 23 metabolites (SDM1) and one containing 46 (SDM2). To evaluate the viability of the SDM, we examined the growth of 30 phylogenetically diverse soil bacterial isolates from the ORFRC field site. The simpler SDM1 supported the growth of 13 isolates while the more complex SDM2 supported 15 isolates. To investigate SDM1 substrate preferences, one isolate,
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