ArticleScientific reports2018
A novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Coxsackievirus B escapes antiviral CD8Science advances · 2026Article
- Coxsackie B1 virus-like particle vaccine modified to exclude a highly conserved immunoreactive region from the capsid induces potent neutralizing antibodies and protects against infection in mice.Journal of biomedical science · 2025Article
- Major Group-B Enterovirus populations deleted in the noncoding 5' region of genomic RNA modulate activation of the type I interferon pathway in cardiomyocytes and induce myocarditis.PLoS pathogens · 2024Article
- Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8Science advances · 2024Article
- Neutralizing antibody landscape of the non-polio Enteroviruses and future strategy.Frontiers in immunology · 2024Review
- Assessment of Enterovirus Antibodies during Early Childhood Using a Multiplex Immunoassay.Microbiology spectrum · 2023Article
- Identification of a Conserved, Linear Epitope on VP3 ofViruses · 2023Article
- Persistent coxsackievirus B1 infection triggers extensive changes in the transcriptome of human pancreatic ductal cells.iScience · 2022Article
- Detection of Viral -RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues.Microorganisms · 2020Article
- Genetic Adaptation of Coxsackievirus B1 during Persistent Infection in Pancreatic Cells.Microorganisms · 2020Article
- Multiplexed High-Throughput Serological Assay for Human Enteroviruses.Microorganisms · 2020Article
- A hexavalent Coxsackievirus B vaccine is highly immunogenic and has a strong protective capacity in mice and nonhuman primates.Science advances · 2020Article
- Antibody Responses against Enterovirus Proteases are Potential Markers for an Acute Infection.Viruses · 2020Article
- Large enteroviral vaccination studies to prevent type 1 diabetes should be well founded and rely on scientific evidence.Diabetologia · 2019Article
- Enterovirus infection and type 1 diabetes: unraveling the crime scene.Clinical and experimental immunology · 2019Review
- Enteroviral infections in the pathogenesis of type 1 diabetes: new insights for therapeutic intervention.Current opinion in pharmacology · 2018Review
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enteroviruses (EVs) are common RNA viruses that cause diseases ranging from rash to paralytic poliomyelitis. For example, EV-A and EV-C viruses cause hand-foot and mouth disease and EV-B viruses cause encephalitis and myocarditis, which can result in severe morbidity and mortality. While new vaccines and treatments for EVs are under development, methods for studying and diagnosing EV infections are still limited and therefore new diagnostic tools are required. Our aim was to produce and characterize new antibodies that work in multiple applications and detect EVs in tissues and in vitro. Rats were immunized with Coxsackievirus B1 capsid protein VP1 and hybridomas were produced. Hybridoma clones were selected based on their reactivity in different immunoassays. The most promising clone, 3A6, was characterized and it performed well in multiple techniques including ELISA, immunoelectron microscopy, immunocyto- and histochemistry and in Western blotting, detecting EVs in infected cells and tissues. It recognized several EV-Bs and also the EV-C representative Poliovirus 3, making it a broad-spectrum EV specific antibody. The 3A6 rat monoclonal antibody can help to overcome some of the challenges faced with commonly used EV antibodies: it enables simultaneous use of mouse-derived antibodies in double staining and it is useful in murine models.
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Registered trials
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