ArticleProceedings of the National Academy of Sciences of the United States of America2018
In situ detection of live-to-dead bacteria ratio after inactivation by means of synchronous fluorescence and PCA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 52 citations in OpenAlex.
- Xist condensates: perspectives for therapeutic intervention.Genome biology · 2025Review
- Autofluorescence-guided Removal of Bacterial Biofilms from Tissues Using Cold Atmospheric Pressure Plasma (CAP).IEEE transactions on radiation and plasma medical sciences · 2024Article
- On the Use of the Intrinsic DNA Fluorescence for Monitoring Its Damage: A Contribution from Fundamental Studies.ACS omega · 2024Review
- UV-Induced Spectral and Morphological Changes in Bacterial Spores for Inactivation Assessment.The journal of physical chemistry. B · 2024Article
- Multispectral Portable Fibre-Optic Reflectometer for the Classification of the Origin of Chicken Eggshells in the Case ofSensors (Basel, Switzerland) · 2022Article
- Population genetic and biophysical evidences reveal that purifying selection shapes the genetic landscape of Plasmodium falciparum RH ligands in Chhattisgarh and West Bengal, India.Malaria journal · 2020Article
- Contamination detection by optical measurements in a real-life environment: A hospital case study.Journal of biophotonics · 2020Article
- A tryptophan synchronous and normal fluorescence study on bacteria inactivation mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Spectroscopic analysis of bacterial photoreactivation.Photochemistry and photobiologyArticle
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The determination of live and dead bacteria is of considerable significance for preventing health care-associated infection in hospitals, field clinics, and other areas. In this study, the viable (live) and nonviable (dead) bacteria in a sample were determined by means of their fluorescence spectra and principal component analysis (PCA). Data obtained in this study show that it is possible to identify bacteria strains and determine the live/dead ratio after UV light inactivation and antibiotic treatment, in situ, within minutes. In addition, synchronous fluorescence scans enable the identification of bacterial components such as tryptophan, tyrosine, and DNA. Compared with the time-consuming plating and culturing methods, this study renders a means for rapid detection and determination of live and dead bacteria.
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