ArticleMethods in cell biology2018
Methods for the visualization and analysis of extracellular matrix protein structure and degradation.
Article in Methods in cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 69 citations in OpenAlex.
- Histopathological and immunohistochemical characterization of pulp tissue reaction to ACTIVA BioACTIVE base/liner in primary teeth pulpotomy: a randomized clinical trial.BMC oral health · 2025Trial
- Hydrolyzed Collagen from Salmon Skin Mitigates L-NAME-Induced Hypertension in Rats by Attenuating Oxidative Stress and Inflammation and Improving Vascular Remodeling.International journal of molecular sciences · 2026Article
- From Hematoxylin and Eosin to Masson's Trichrome: A Comprehensive Framework for Virtual Stain Transformation in Chronic Liver Disease Diagnosis.Diagnostics (Basel, Switzerland) · 2026Article
- Article
- MALDI mass spectrometry imaging of extracellular matrix proteins.The Analyst · 2025Review
- Advances in abiotic tissue-based biomaterials: A focus on decellularization and devitalization techniques.Materials today. Bio · 2025Article
- Durotaxis and extracellular matrix degradation promote the clustering of cancer cells.iScience · 2025Article
- Decellularization of camel corneas for tissue engineering applications.International ophthalmology · 2025Article
- Advances in Extracellular Matrix-Associated Diagnostics and Therapeutics.Journal of clinical medicine · 2025Review
- Leveraging Optical Anisotropy of the Morpho Butterfly Wing for Quantitative, Stain-Free, and Contact-Free Assessment of Biological Tissue Microstructures.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- High-Throughput Single-Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC).Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Decellularization and Enzymatic Digestion Methods to Enhance ECM Protein Detection via MALDI-MS Imaging.Analytical chemistry · 2025Article
- Recent Updates on Protease Zymography.Methods in molecular biology (Clifton, N.J.) · 2025Article
- FIRM image analysis: A machine learning workflow for quantifying extracellular matrix components from electron microscopy images.PloS one · 2025Article
- Distinct mesenchymal cell states mediate prostate cancer progression.Nature communications · 2024Article
- In-vivo studies of targeted and localized cancer drug release from microporous poly-di-methyl-siloxane (PDMS) devices for the treatment of triple negative breast cancer.Scientific reports · 2024Article
- Biomechanical effects of hemin and sildenafil treatments on the aortic wall of chronic-hypoxic lambs.Frontiers in bioengineering and biotechnology · 2024Article
- Modeling the extracellular matrix in cell migration and morphogenesis: a guide for the curious biologist.Frontiers in cell and developmental biology · 2024Review
- Wound management, healing, and early prosthetic rehabilitation: Part 2 - A scoping review of physical biomarkers.Canadian prosthetics & orthotics journal · 2024Review
- Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem-Cell Niches for In Vitro Models.Advanced materials (Deerfield Beach, Fla.) · 2023Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
This chapter highlights methods for visualization and analysis of extracellular matrix (ECM) proteins, with particular emphasis on collagen type I, the most abundant protein in mammals. Protocols described range from advanced imaging of complex in vivo matrices to simple biochemical analysis of individual ECM proteins. The first section of this chapter describes common methods to image ECM components and includes protocols for second harmonic generation, scanning electron microscopy, and several histological methods of ECM localization and degradation analysis, including immunohistochemistry, Trichrome staining, and in situ zymography. The second section of this chapter details both a common transwell invasion assay and a novel live imaging method to investigate cellular behavior with respect to collagen and other ECM proteins of interest. The final section consists of common electrophoresis-based biochemical methods that are used in analysis of ECM proteins. Use of the methods described herein will enable researchers to gain a greater understanding of the role of ECM structure and degradation in development and matrix-related diseases such as cancer and connective tissue disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.