Evidence map›Paper›PMID 29310787›Full record

ArticleMethods in cell biology2018

Thrombospondins: Purification of human platelet thrombospondin-1.

John M Sipes, Joanne E Murphy-Ullrich, David D Roberts

Abstract read
In one paragraph

Article in Methods in cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Review
  3. International journal of molecular sciences · 2024
    Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Review
  10. Matrix mechanotransduction mediated by thrombospondin-1/integrin/YAP in the vascular remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

John M SipesLaboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.
Joanne E Murphy-UllrichUniversity of Alabama at Birmingham, Birmingham, AL, United States. Electronic address: jmurphy@uabmc.edu.
David D RobertsLaboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States. Electronic address: droberts@helix.nih.gov.
National Cancer Institute · USUniversity of Alabama at Birmingham · US

Funding

Cellular Interactions with ThrombospondinZIASC009172 · NCI · DIVISION OF CLINICAL SCIENCES - NCI · PI ROBERTS, DAVID D · 2009 to 2024
$17.9M
The thrombospondin1-TGF-beta axis in multiple myelomaR01CA175012 · NCI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI MURPHY-ULLRICH, JOANNE E · 2014 to 2018
$3.0M
Intramural NIH HHS ZIA SC009172NCI NIH HHS R01 CA175012
6 · The paper itself

Abstract

Thrombospondins are a family of five secreted proteins that have diverse roles in modulating cellular function. Thrombospondins-1 and 2 were identified as matricellular proteins based on their functional roles combined with their transient appearance or accumulation in extracellular matrix at specific times during development and in response to injury or stress in mature tissues. Thrombospondin-1 is a major component of platelet α-granules, which provides a convenient source for purification of the protein. Methods are described to prepare thrombospondin-1 from human platelets in a biologically active form with minimal degradation or contamination with other platelet proteins. A nondenaturing method is described for removing bound transforming growth factor-β1.

Indexed as

Blood PlateletsChromatography, AffinityExtracellular MatrixFibronectinsHeparinHumansThrombospondin 1Transforming Growth Factor beta1FibronectinsHeparinTGFB1 protein, humanThrombospondin 1thrombospondin-1, humanTransforming Growth Factor beta1Affinity chromatographyFibronectinHeparinPlateletsThrombospondin-1ThrombospondinsTransforming growth factor-β

Identifiers

PMID29310787
PMCPMC6642635
OpenAlexW2767660461

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.