ArticleMethods in cell biology2018
The biochemistry and immunohistochemistry of versican.
Article in Methods in cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- The Role of DNA Degradation in the Estimation of Post-Mortem Interval: A Systematic Review of the Current Literature.International journal of molecular sciences · 2020Pooled it
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- Multiomics analysis of platelet-rich plasma promoting biological performance of mesenchymal stem cells.BMC genomics · 2024Article
- Focal clusters of peri-synaptic matrix contribute to activity-dependent plasticity and memory in mice.Cell reports · 2024Article
- Absolute quantification of neuromelanin in formalin-fixed human brains using absorbance spectrophotometry.PloS one · 2023Article
- Spatiotemporal microvascular changes following contusive spinal cord injury.Frontiers in neuroanatomy · 2023Article
- Biochemical, biophysical, and immunological characterization of respiratory secretions in severe SARS-CoV-2 infections.JCI insight · 2022Article
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- Versican promotes T helper 17 cytotoxic inflammation and impedes oligodendrocyte precursor cell remyelination.Nature communications · 2022Article
- Biochemical, Biophysical, and Immunological Characterization of Respiratory Secretions in Severe SARS-CoV-2 (COVID-19) Infections.medRxiv : the preprint server for health sciences · 2022Article
- The Use of Quantitative Digital Pathology to Measure Proteoglycan and Glycosaminoglycan Expression and Accumulation in Healthy and Diseased Tissues.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021Review
- Single-cell RNA sequencing deconvolutes theInternational journal of biological sciences · 2021Article
- A Role for HAPLN1 During Phenotypic Modulation of Human Lung Fibroblasts In Vitro.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- Versican-A Critical Extracellular Matrix Regulator of Immunity and Inflammation.Frontiers in immunology · 2020Review
- Quantification of the regional bioarchitecture in the human aorta.Journal of anatomy · 2020Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Versican is a chondroitin sulfate proteoglycan found in the extracellular matrix that is important for changes in cell phenotype associated with development and disease. Versican has been shown to be involved in cardiovascular disorders, as well as lung disease and fibrosis, inflammatory bowel disease, cancer, and several other diseases that have an inflammatory component. Versican was first identified as a fibroblast proteoglycan and forms large multimolecular complexes with hyaluronan and other components of the provisional matrix during wound healing and inflammation. The biology of versican has been well studied. Versican plays a major role in embryogenesis, particularly heart formation, where versican deletion proves lethal. The ability to purify versican to characterize and to use in experimental systems is vital to defining its role in development and disease. Protein expression systems have proven challenging to obtain milligram quantities of full-length versican. Here, we describe proteoglycan biochemical purification techniques that have been developed by others, but which we have adapted to use with our source tissues and cells. We also include methods for immunohistochemical localization and quantitation of versican in tissue sections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.