ReviewMicrobial genomics2018
Culture-independent approaches to chlamydial genomics.
Review in Microbial genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Whole-genome sequencing-based pathogen characterization for streptococcal infection directly from positive blood culture samples.Journal of clinical microbiology · 2026Article
- Nanopore- and AI-empowered microbial viability inference.GigaScience · 2025Article
- StrengtheningJournal of clinical microbiology · 2024Article
- Whole-genome sequencing ofMicrobial genomics · 2023Article
- Gene gain facilitated endosymbiotic evolution of Chlamydiae.Nature microbiology · 2023Article
- Genome organization and genomics inFrontiers in cellular and infection microbiology · 2023Review
- One clone to rule them all: Culture-independent genomics ofMicrobial genomics · 2022Article
- Ecology and evolution of chlamydial symbionts of arthropods.ISME communications · 2022Article
- Evaluation of Urethrotropic-Clade Meningococcal Infection by Urine Metagenomic Shotgun Sequencing.Journal of clinical microbiology · 2022Article
- Specific high-resolution scheme to improve understanding of the spatio-temporal dispersion of lymphogranuloma venereum epidemic.Frontiers in microbiology · 2022Article
- Completing the Genome Sequence ofPathogens (Basel, Switzerland) · 2021Article
- Pangenomics reveals alternative environmental lifestyles among chlamydiae.Nature communications · 2021Article
- Whole-Genome Enrichment and Sequencing of Chlamydia trachomatis Directly from Patient Clinical Vaginal and Rectal Swabs.mSphere · 2021Article
- Chlamydiae - What's New?Journal of veterinary research · 2020Article
- Real-Time Selective Sequencing with RUBRIC: Read Until with Basecall and Reference-Informed Criteria.Scientific reports · 2019Article
- Metagenomic Analysis of Fish-Associated Ca. Parilichlamydiaceae Reveals Striking Metabolic Similarities to the Terrestrial Chlamydiaceae.Genome biology and evolution · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The expanding field of bacterial genomics has revolutionized our understanding of microbial diversity, biology and phylogeny. For most species, DNA extracted from culture material is used as the template for genome sequencing; however, the majority of microbes are actually uncultivable, and others, such as obligate intracellular bacteria, require laborious tissue culture to yield sufficient genomic material for sequencing. Chlamydiae are one such group of obligate intracellular microbes whose characterization has been hampered by this requirement. To circumvent these challenges, researchers have developed culture-independent sample preparation methods that can be applied to the sample directly or to genomic material extracted from the sample. These methods, which encompass both targeted [immunomagnetic separation-multiple displacement amplification (IMS-MDA) and sequence capture] and non-targeted approaches (host methylated DNA depletion-microbial DNA enrichment and cell-sorting-MDA), have been applied to a range of clinical and environmental samples to generate whole genomes of novel chlamydial species and strains. This review aims to provide an overview of the application, advantages and limitations of these targeted and non-targeted approaches in the chlamydial context. The methods discussed also have broad application to other obligate intracellular bacteria or clinical and environmental samples.
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Registered trials
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