Evidence map›Paper›PMID 29310075›Full record

ArticleRedox biology2018

A single-cysteine mutant and chimeras of essential Leishmania Erv can complement the loss of Erv1 but not of Mia40 in yeast.

Sandra Specht, Linda Liedgens, Margarida Duarte, Alexandra Stiegler, Ulrike Wirth, Maike Eberhardt, Ana Tomás, Kai Hell, Marcel Deponte

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Redox biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Oxidation ofAntioxidants (Basel, Switzerland) · 2023
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  3. Parasite powerhouse: A review of the Toxoplasma gondii mitochondrion.The Journal of eukaryotic microbiology · 2022
    Review
  4. Microbiology spectrum · 2021
    Article
  5. Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Sandra SpechtDepartment of Parasitology, Ruprecht-Karls University, D-69120 Heidelberg, Germany.
Linda LiedgensDepartment of Parasitology, Ruprecht-Karls University, D-69120 Heidelberg, Germany.
Margarida Duartei3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4200, Portugal; Institute for Molecular and Cell Biology - IBMC, Universidade do Porto, 4200-135 Porto, Portugal.
Alexandra StieglerBiomedical Center Munich - Physiological Chemistry, Ludwig-Maximilians University, D-82152 Planegg, Martinsried, Germany.
Ulrike WirthBiomedical Center Munich - Physiological Chemistry, Ludwig-Maximilians University, D-82152 Planegg, Martinsried, Germany.
Maike EberhardtDepartment of Parasitology, Ruprecht-Karls University, D-69120 Heidelberg, Germany.
Ana Tomási3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4200, Portugal; Institute for Molecular and Cell Biology - IBMC, Universidade do Porto, 4200-135 Porto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto 4050-313, Portugal.
Kai HellBiomedical Center Munich - Physiological Chemistry, Ludwig-Maximilians University, D-82152 Planegg, Martinsried, Germany. Electronic address: kai.hell@bmc.med.lmu.de.
Marcel DeponteDepartment of Parasitology, Ruprecht-Karls University, D-69120 Heidelberg, Germany; Faculty of Chemistry - Biochemistry, Kaiserslautern University, D-67663 Kaiserslautern, Germany. Electronic address: deponte@chemie.uni-kl.de.
Heidelberg University · DELudwig-Maximilians-Universität München · DEi3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto · PTUniversidade do Porto · PTUniversity of Kaiserslautern · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mia40/CHCHD4 and Erv1/ALR are essential for oxidative protein folding in the mitochondrial intermembrane space of yeast and mammals. In contrast, many protists, including important apicomplexan and kinetoplastid parasites, lack Mia40. Furthermore, the Erv homolog of the model parasite Leishmania tarentolae (LtErv) was shown to be incompatible with Saccharomyces cerevisiae Mia40 (ScMia40). Here we addressed structure-function relationships of ScErv1 and LtErv as well as their compatibility with the oxidative protein folding system in yeast using chimeric, truncated, and mutant Erv constructs. Chimeras between the N-terminal arm of ScErv1 and a variety of truncated LtErv constructs were able to rescue yeast cells that lack ScErv1. Yeast cells were also viable when only a single cysteine residue was replaced in LtErv

Indexed as

AnimalsCysteineDisease Models, AnimalHumansLeishmaniaLeishmaniasisMitochondriaMitochondrial Membrane Transport ProteinsMitochondrial Precursor Protein Import Complex ProteinsMitochondrial ProteinsMutationOxidoreductases Acting on Sulfur Group DonorsProtein DomainsProtein FoldingProtozoan ProteinsSaccharomyces cerevisiaeCysteineERV1 protein, S cerevisiaeMIA40 protein, S cerevisiaeMitochondrial Membrane Transport ProteinsMitochondrial Precursor Protein Import Complex ProteinsMitochondrial ProteinsOxidoreductases Acting on Sulfur Group DonorsProtozoan ProteinsSaccharomyces cerevisiae ProteinsALRCHCHD4ErvIntermembrane spaceLeishmaniaMia40MitochondriaOxidative protein folding

Identifiers

PMID29310075
PMCPMC5760468
OpenAlexW2781111844

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.