ArticleNucleic acids research2018
A fungal Argonaute interferes with RNA interference.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- Fungal Argonaute proteins act in bidirectional cross-kingdom RNA interference during plant infection.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Harnessing RNA interference for the control of Fusarium species: A critical review.Molecular plant pathology · 2024Review
- Argonaute-independent, Dicer-dependent antiviral defense against RNA viruses.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Advances in UnderstandingGenes · 2024Review
- Identification of Mycoviruses in the Pathogens of Fragrant Pear Valsa Canker from Xinjiang in China.Viruses · 2024Article
- Function and regulation of plant ARGONAUTE proteins in response to environmental challenges: a review.PeerJ · 2024Review
- Horizontally Transferred DNA in the Genome of the Fungus Pyricularia oryzae is Associated With Repressive Histone Modifications.Molecular biology and evolution · 2023Article
- Experimental verification of strain-dependent relationship between mycovirus and its fungal host.iScience · 2023Article
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- Article
- RNA interference ofFrontiers in microbiology · 2023Article
- Interference of Small RNAs inJournal of fungi (Basel, Switzerland) · 2022Article
- Molecular mechanisms underlying host-induced gene silencing.The Plant cell · 2022Review
- FoQDE2-dependent milRNA promotes Fusarium oxysporum f. sp. cubense virulence by silencing a glycosyl hydrolase coding gene expression.PLoS pathogens · 2022Article
- Exploring the Effectiveness and Durability of Trans-Kingdom Silencing of Fungal Genes in the Vascular PathogenInternational journal of molecular sciences · 2022Article
- Comparative profiling of canonical and non-canonical small RNAs in the rice blast fungus,Frontiers in microbiology · 2022Article
- Mutations in PpAGO3 Lead to Enhanced Virulence ofFrontiers in microbiology · 2022Article
- Mycovirus-encoded suppressors of RNA silencing: Possible allies or enemies in the use of RNAi to control fungal disease in crops.Frontiers in fungal biology · 2022Review
- Copy number-dependent DNA methylation of the Pyricularia oryzae MAGGY retrotransposon is triggered by DNA damage.Communications biology · 2021Article
- Identification of an RNA Silencing Suppressor Encoded by a Symptomless Fungal Hypovirus, Cryphonectria Hypovirus 4.Biology · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein-sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2, one of the three AGO genes in the fungus Pyricularia oryzae (Magnaporthe oryzae) interferes with RNAi. Gene knockout (KO) studies revealed that MoAGO1 and MoAGO3 additively or redundantly played roles in hairpin RNA- and retrotransposon (MAGGY)-triggered RNAi while, surprisingly, the KO mutants of MoAGO2 (Δmoago2) showed elevated levels of gene silencing. Consistently, transcript levels of MAGGY and mycoviruses were drastically reduced in Δmoago2, supporting the idea that MoAGO2 impeded RNAi against the parasitic elements. Deep sequencing analysis revealed that repeat- and mycovirus-derived small interfering RNAs were mainly associated with MoAGO2 and MoAGO3, and their populations were very similar based on their size distribution patterns and positional base preference. Site-directed mutagenesis studies indicated that sRNA binding but not slicer activity of MoAGO2 was essential for the ability to diminish the efficacy of RNAi. Overall, these results suggest a possible interplay between distinct sRNA-mediated gene regulation pathways through a competition for sRNA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.