ArticleJournal of experimental botany2018
Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)Autophagy · 2021Guideline
- Heat and Drought Co-Stress During Microgametogenesis Altered Pollen Development, Fertility and Grain Production in Two-Rowed Winter Barley.Plants (Basel, Switzerland) · 2026Article
- From pollen precursor to totipotent embryo: a model system for plant cell developmental plasticity.Plant cell reports · 2026Review
- 5-azacytidine affects triticale microspore embryogenesis and is associated with modulation of reactive oxygen species, cysteine protease profiles, and programmed cell death.BMC plant biology · 2026Article
- NBR1-Mediated Selective Autophagy in Plant Development and Stress Responses.Plants (Basel, Switzerland) · 2026Review
- Platelet Autophagy as a Druggable Intracellular Pathway: Therapeutic Opportunities in Thromboinflammatory Diseases.Pharmaceutics · 2026Review
- Monitoring Barley Microspore Embryogenesis: In Vitro Culture and Immunolocalization Analyses.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Plant caspase-like proteins: from function identification to application in winter rapeseed genetic breeding.Frontiers in plant science · 2026Review
- Molecular Insights into Microspore Embryogenesis: Transcriptomic Dynamics in Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2026Review
- SlATG8f modulates tomato thermotolerance and fruit quality, correlating with changes in autophagy and heat shock-related genes.PloS one · 2026Article
- Induction of microspore embryogenesis in bread wheat by mannitol pre-treatment is associated with the disruption of endogenous hormone balance and substantial accumulation of auxins.BMC plant biology · 2025Article
- Unraveling the role of autophagy and antioxidants in anther and pistil responses to heat stress in rapeseed (Brassica napus L.).Plant cell reports · 2025Article
- Deciphering the link: ferroptosis and its role in glioma.Frontiers in immunology · 2024Review
- Plant cathepsin B, a versatile protease.Frontiers in plant science · 2024Review
- Transcriptomics integrated with metabolomics reveals the effect of cold stress on rice microspores.BMC plant biology · 2023Article
- The Roads to Haploid Embryogenesis.Plants (Basel, Switzerland) · 2023Review
- Review
- Ferroptosis and Its Potential Role in Glioma: From Molecular Mechanisms to Therapeutic Opportunities.Antioxidants (Basel, Switzerland) · 2022Review
- Autophagy in the Lifetime of Plants: From Seed to Seed.International journal of molecular sciences · 2022Review
- The Key Role of Lysosomal Protease Cathepsins in Viral Infections.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microspores are reprogrammed towards embryogenesis by stress. Many microspores die after this stress, limiting the efficiency of microspore embryogenesis. Autophagy is a degradation pathway that plays critical roles in stress response and cell death. In animals, cathepsins have an integral role in autophagy by degrading autophagic material; less is known in plants. Plant cathepsins are papain-like C1A cysteine proteases involved in many physiological processes, including programmed cell death. We have analysed the involvement of autophagy in cell death, in relation to cathepsin activation, during stress-induced microspore embryogenesis in Hordeum vulgare. After stress, reactive oxygen species (ROS) and cell death increased and autophagy was activated, including HvATG5 and HvATG6 up-regulation and increase of ATG5, ATG8, and autophagosomes. Concomitantly, cathepsin L/F-, B-, and H-like activities were induced, cathepsin-like genes HvPap-1 and HvPap-6 were up-regulated, and HvPap-1, HvPap-6, and HvPap-19 proteins increased and localized in the cytoplasm, resembling autophagy structures. Inhibitors of autophagy and cysteine proteases reduced cell death and promoted embryogenesis. The findings reveal a role for autophagy in stress-induced cell death during microspore embryogenesis, and the participation of cathepsins. Similar patterns of activation, expression, and localization suggest a possible connection between cathepsins and autophagy. The results open up new possibilities to enhance microspore embryogenesis efficiency with autophagy and/or cysteine protease modulators.
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