ArticleNature chemical biology2018
Employing a biochemical protecting group for a sustainable indigo dyeing strategy.
Article in Nature chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed, 212 citations in OpenAlex.
- Antibiotic- and inducer-free biosynthesis of indican from crude glycerol using engineered probiotic Escherichia coli Nissle 1917.Bioresources and bioprocessing · 2026Article
- Process Intensification Sonocatalytic Degradation of Malachite Green Wastewater over Hydrothermally Engineered M-ZLT@CoMolecules (Basel, Switzerland) · 2026Article
- Designing an ecofriendly catalyst for a sustainable use of water resources.National science review · 2026Article
- Engineering artificial biosynthetic pathway enables simultaneous production and in-situ bio-dyeing of indigoids for textiles.Nature communications · 2025Article
- Developing Quorum Sensing-Based Collaborative Dynamic Control System in Halomonas TD01.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Microbial Pathways for Cost-Effective Low-Carbon Renewable Indigoidine.ACS sustainable chemistry & engineering · 2025Article
- Structural Insights into the Substrate Recognition of Ginsenoside Glycosyltransferase Pq3-O-UGT2.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- PtBGL: a cost-effective alternative to GUS reporter with applications in plant imprint dyeing.Frontiers in plant science · 2025Article
- Finding of the positive impact of glucose on the production of indican over indigo in engineered Escherichia coli.Journal of industrial microbiology & biotechnology · 2024Article
- Plants Utilize a Protection/Deprotection Strategy in Limonoid Biosynthesis: A "Missing Link" Carboxylesterase Boosts Yields and Provides Insights into Furan Formation.Journal of the American Chemical Society · 2024Article
- Study of Woad (Molecules (Basel, Switzerland) · 2024Article
- Metagenomic and metabolite analysis reveals microbial community and metabolite dynamics in fermentedHeliyon · 2024Article
- Functional characterization, structural basis, and protein engineering of a rare flavonoid 2'-Acta pharmaceutica Sinica. B · 2024Article
- Enzymatic glycosylation of aloesone performed by plant UDP-dependent glycosyltransferases.Glycobiology · 2024Article
- A growth selection system for sucrose synthases (SuSy): design and test.AMB Express · 2024Article
- Synthetic microbe-to-plant communication channels.Nature communications · 2024Article
- Chemoenzymatic indican for light-driven denim dyeing.Nature communications · 2024Article
- Indigo production goes green: a review on opportunities and challenges of fermentative production.World journal of microbiology & biotechnology · 2024Review
- Harnessing Plant Sugar Metabolism for Glycoengineering.Biology · 2023Article
- Insights into the missing apiosylation step in flavonoid apiosides biosynthesis of Leguminosae plants.Nature communications · 2023Article
Corrections and comments
- Commented on by
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Indigo is an ancient dye uniquely capable of producing the signature tones in blue denim; however, the dyeing process requires chemical steps that are environmentally damaging. We describe a sustainable dyeing strategy that not only circumvents the use of toxic reagents for indigo chemical synthesis but also removes the need for a reducing agent for dye solubilization. This strategy utilizes a glucose moiety as a biochemical protecting group to stabilize the reactive indigo precursor indoxyl to form indican, preventing spontaneous oxidation to crystalline indigo during microbial fermentation. Application of a β-glucosidase removes the protecting group from indican, resulting in indigo crystal formation in the cotton fibers. We identified the gene coding for the glucosyltransferase PtUGT1 from the indigo plant Polygonum tinctorium and solved the structure of PtUGT1. Heterologous expression of PtUGT1 in Escherichia coli supported high indican conversion, and biosynthesized indican was used to dye cotton swatches and a garment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.