ArticleCell journal2018
Direct Coculture of Human Chondrocytes and Synovium-Derived Stem Cells Enhances In Vitro Chondrogenesis.
Article in Cell journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Harnessing organoid technology to model autoimmune pathways in rheumatological and inflammatory disorders.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- Human adipose and synovial-derived MSCs synergistically attenuate osteoarthritis by promoting chondrocyte autophagy through FoxO1 signaling.Stem cell research & therapy · 2024Article
- Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment.Cell communication and signaling : CCS · 2024Review
- 3D-printed PCL scaffolds with anatomy-inspired bionic stratified structures for the treatment of growth plate injuries.Materials today. Bio · 2023Article
- Review
- Cartilage Tissue Engineering: An Introduction.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Co-culture pellet of human Wharton's jelly mesenchymal stem cells and rat costal chondrocytes as a candidate for articular cartilage regeneration: in vitro and in vivo study.Stem cell research & therapy · 2022Article
- Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs.Journal of orthopaedic translation · 2022Article
- Probing Multicellular Tissue Fusion of Cocultured Spheroids-A 3D-Bioassembly Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Article
- Effect of a subset of adipose-derived stem cells isolated with liposome magnetic beads to promote cartilage repair.Journal of cellular and molecular medicine · 2021Article
- Heterogeneity of mesenchymal stem cells in cartilage regeneration: from characterization to application.NPJ Regenerative medicine · 2021Review
- In Vitro Human Joint Models Combining Advanced 3D Cell Culture and Cutting-Edge 3D Bioprinting Technologies.Cells · 2021Review
- Feasibility of Human Platelet Lysate as an Alternative to Foetal Bovine Serum for In Vitro Expansion of Chondrocytes.International journal of molecular sciences · 2021Review
- Scaffold-Based Tissue Engineering Strategies for Osteochondral Repair.Frontiers in bioengineering and biotechnology · 2021Review
- A Human Osteochondral Tissue Model Mimicking Cytokine-Induced Key Features of Arthritis In Vitro.International journal of molecular sciences · 2020Article
- Utility of direct 3D co-culture model for chondrogenic differentiation of mesenchymal stem cells on hyaluronan scaffold (Hyaff-11).Regenerative biomaterials · 2020Article
- The use of large animals to facilitate the process of MSC going from laboratory to patient-'bench to bedside'.Cell biology and toxicology · 2020Review
- Chondrocyte Culture Parameters for Matrix-Assisted Autologous Chondrocyte Implantation Affect Catabolism and Inflammation in a Rabbit Model.International journal of molecular sciences · 2019Article
- Short exposure to tranexamic acid does not affect, in vitro, the viability of human chondrocytes.European journal of medical research · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesCoculture of chondrocytes and mesenchymal stem cells (MSCs) has been developed as a strategy to overcome the dedifferentiation of chondrocytes during in vitro expansion in autologous chondrocyte transplantation. Synovium-derived stem cells (SDSCs) can be a promising cell source for coculture due to their superior chondrogenic potential compared to other MSCs and easy accessibility without donor site morbidity. However, studies on coculture of chondrocytes and SDSCs are very limited. The aim of this study was to investigate whether direct coculture of human chondrocytes and SDSCs could enhance chondrogenesis compared to monoculture of each cell. MATERIALS AND
methodsIn this experimental study, passage 2 chondrocytes and SDSCs were directly cocultured using different ratios of chondrocytes to SDSCs (3:1, 1:1, or 1:3). glycosaminoglycan (GAG) synthetic activity was assessed using GAG assays and Safranin-O staining. Expression of chondrogenesis-related genes (collagen types I, II, X, Aggrecan, and Sox-9) were analyzed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry staining.
resultsGAG/DNA ratios in 1:1 and 1:3 coculture groups were significantly increased compared to those in the chondrocyte and SDSC monoculture groups. Type II collagen and SOX-9 were significantly upregulated in the 1:1 coculture group compared to those in the chondrocyte and SDSC monoculture groups. On the other hand, osteogenic marker (type I collagen) and hypertrophic marker (type X collagen) were significantly downregulated in the coculture groups compared to those in the SDSC monoculture group.
conclusionsDirect coculture of human chondrocytes and SDSCs significantly enhanced chondrogenic potential, especially at a ratio of 1:1, compared to chondrocyte or SDSC monocultures.
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