Evidence map›Paper›PMID 29307513›Full record

ArticleMolecular cell2018

Visualizing Dynamics of Cell Signaling In Vivo with a Phase Separation-Based Kinase Reporter.

Qiang Zhang, Hai Huang, Luqing Zhang, Roland Wu, Chan-I Chung, Shao-Qing Zhang, Joaquim Torra, Antonino Schepis, Shaun R Coughlin, Thomas B Kornberg and 1 more

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 87 papers.

0numbers the graph read from it
0cells of the map it votes in
87citing papers in PubMed
6.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

87 citing papers in PubMed, 157 citations in OpenAlex.

  1. Review
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  8. Active zone plasticity couples sleep need to presynaptic hypophosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2026
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  9. A Constellation of Fluorescent Biosensors to Illuminate the Galaxy of Protein Kinases.Chembiochem : a European journal of chemical biology · 2026
    Review
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  11. Chemical reviews · 2026
    Review
  12. Article
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  18. Controllable gap junctions by vitamin BProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  19. Article
  20. Article

27 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Qiang ZhangDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94143, USA; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Hai HuangCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Luqing ZhangCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Roland WuCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Chan-I ChungDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94143, USA; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Shao-Qing ZhangCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Joaquim TorraDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94143, USA; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA; Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain.
Antonino SchepisCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Shaun R CoughlinCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Thomas B KornbergCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Xiaokun ShuDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94143, USA; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: xiaokun.shu@ucsf.edu.
University of California, San Francisco · USUniversitat Ramon Llull · ES

Funding

MOLECULAR AND CELLULAR BASIS OF CARDIOVASCULAR DISEASET32HL007731 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Brian L Black · 1992 to 2026
$12.4M
Molecular Mechanisms in DevelopmentR35GM122548 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI THOMAS B. KORNBERG · 2017 to 2026
$8.8M
MOLECULAR GENETICS OF CARDIAC CELL DIFFERENTIATIONR01HL054737 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI COUGHLIN, SHAUN R. · 1996 to 2015
$5.4M
Structural Basis of Protease-Activated Receptor FunctionR01HL121387 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI COUGHLIN, SHAUN R., KOBILKA, BRIAN K · 2014 to 2017
$2.9M
New principle-based technologies for identifying transient protein interactionsDP2GM105446 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SHU, XIAOKUN · 2012 to 2012
$2.4M
NHLBI NIH HHS R01 HL054737NHLBI NIH HHS R01 HL121387NHLBI NIH HHS T32 HL007731NIGMS NIH HHS DP2 GM105446NIGMS NIH HHS R35 GM122548
6 · The paper itself

Abstract

Visualizing dynamics of kinase activity in living animals is essential for mechanistic understanding of cell and developmental biology. We describe GFP-based kinase reporters that phase-separate upon kinase activation via multivalent protein-protein interactions, forming intensively fluorescent droplets. Called SPARK (separation of phases-based activity reporter of kinase), these reporters have large dynamic range (fluorescence change), high brightness, fast kinetics, and are reversible. The SPARK-based protein kinase A (PKA) reporter reveals oscillatory dynamics of PKA activities upon G protein-coupled receptor activation. The SPARK-based extracellular signal-regulated kinase (ERK) reporter unveils transient dynamics of ERK activity during tracheal metamorphosis in live Drosophila. Because of intensive brightness and simple signal pattern, SPARKs allow easy examination of kinase signaling in living animals in a qualitative way. The modular design of SPARK will facilitate development of reporters of other kinases.

Indexed as

AnimalsCyclic AMP-Dependent Protein KinasesDrosophilaEnzyme ActivationExtracellular Signal-Regulated MAP KinasesGreen Fluorescent ProteinsHumansMAP Kinase Signaling SystemOptical ImagingPhosphorylationPhosphotransferasesSignal TransductionCyclic AMP-Dependent Protein KinasesExtracellular Signal-Regulated MAP KinasesGreen Fluorescent ProteinsPhosphotransferasesDrosophilaERKGFPG protein coupled receptorkinasekinase reporterphase separationprotein kinase Aprotein-protein interactionreceptor tyrosine kinase

Identifiers

PMID29307513
PMCPMC5788022
OpenAlexW2782081342

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.