Evidence map›Paper›PMID 29305959›Full record

ArticleInternational journal of antimicrobial agents2018

Enhanced in vitro activity of tigecycline in the presence of chelating agents.

Amelia N Deitchman, Ravi Shankar Prasad Singh, Kenneth H Rand, Hartmut Derendorf

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of antimicrobial agents, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Amelia N DeitchmanUniversity of Florida, Department of Pharmaceutics, P.O. Box 100494, Gainesville, FL 32610, USA.
Ravi Shankar Prasad SinghUniversity of Florida, Department of Pharmaceutics, P.O. Box 100494, Gainesville, FL 32610, USA; Pfizer Inc., 1 Portland Street, Cambridge, MA 02139, USA.
Kenneth H RandUniversity of Florida, Department of Pathology, Immunology and Laboratory Medicine, Box 100275, Gainesville, FL 32610, USA.
Hartmut DerendorfUniversity of Florida, Department of Pharmaceutics, P.O. Box 100494, Gainesville, FL 32610, USA. Electronic address: hartmut@ufl.edu.
University of Florida · USPfizer (United States) · US

Funding

Using social networks to map and evaluate team science across CTSA hubsUL1TR001427 · NCATS · UNIVERSITY OF FLORIDA · PI MITCHELL, DUANE A. · 2015 to 2024
$37.2M
UF Clinical and Translational Science AwardUL1TR000064 · NCATS · UNIVERSITY OF FLORIDA · PI NELSON, DAVID R · 2012 to 2014
$12.2M
NCATS NIH HHS UL1 TR000064NCATS NIH HHS UL1 TR001427
6 · The paper itself

Abstract

The lack of availability of novel antibiotic agents and the rise of resistance to existing therapies has led clinicians to utilise combination therapy to adequately treat bacterial infections. Here we examined how chelators may impact the in vitro activity of tigecycline (TIG) against Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Minimum inhibitory concentrations (MICs) were determined by broth dilution with and without various combinations of chelators (EDTA and other tetracyclines) and metal ions (i.e. calcium, magnesium). Trimethoprim (TMP) was used as a non-chelating control. Addition of metal ions led to increases in MICs, whilst addition of EDTA led to decreases in MICs. The chelating effects of EDTA were reversed by addition of magnesium and most profoundly calcium. Similar effects of EDTA and calcium were observed for tetracycline (TET) and TMP. When other tetracyclines (TET, oxytetracycline (OXY) and chlortetracycline (CHL)) were used as chelators at concentrations below their MICs, TIG MICs decreased for P. aeruginosa but not for E. coli. Some decreases in TIG MICs were observed for K. pneumoniae when TET and CHL were added. A dose-dependent decrease in TIG MIC was observed for TET and was reversed by the addition of calcium. The presence of effects of EDTA and calcium on TMP MICs indicates that mechanisms outside of TIG chelation likely play a role in enhanced activity. Full characterisation of an unexpected interaction such as TIG-TET with different microorganisms could provide valuable insights into the underlying mechanisms and design of physiologically viable chelators as candidates for future combinations regimens.

Indexed as

Anti-Bacterial AgentsCalciumChelating AgentsChlortetracyclineDose-Response Relationship, DrugDrug SynergismEdetic AcidEscherichia coliKlebsiella pneumoniaeMagnesiumMicrobial Sensitivity TestsMinocyclinePseudomonas aeruginosaTetracyclineTigecyclineAnti-Bacterial AgentsCalciumChelating AgentsChlortetracyclineEdetic AcidMagnesiumMinocyclineTetracyclineTigecyclineAntibacterialCalcium chelationDrug combinationsMechanistic investigation

Identifiers

PMID29305959
PMCPMC5940551
OpenAlexW2781480677

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.