Evidence map›Paper›PMID 29305668›Full record

ArticlePharmaceutical research2018

Iloprost Affects Macrophage Activation and CCL2 Concentrations in a Microdialysis Model in Rats.

Kamel Alkhatib, Tina M Poseno, Alda Diaz Perez, Jeannine M Durdik, Julie A Stenken

Abstract read
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Article in Pharmaceutical research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Kamel AlkhatibCell and Molecular Biology Program, University of Arkansas, Fayetteville, Arkansas, 72701, USA.
Tina M PosenoDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas, 72701, USA.
Alda Diaz PerezCell and Molecular Biology Program, University of Arkansas, Fayetteville, Arkansas, 72701, USA.
Jeannine M DurdikCell and Molecular Biology Program, University of Arkansas, Fayetteville, Arkansas, 72701, USA.
Julie A StenkenCell and Molecular Biology Program, University of Arkansas, Fayetteville, Arkansas, 72701, USA. jstenken@uark.edu.
University of Arkansas at Fayetteville · US

Funding

Modulating and Monitoring the Foreign Body Response to ImplantsR01EB014404 · NIBIB · UNIVERSITY OF ARKANSAS AT FAYETTEVILLE · PI STENKEN, JULIE A · 2011 to 2014
$1.3M
NIBIB NIH HHS EB 014404NIBIB NIH HHS R01 EB014404
6 · The paper itself

Abstract

purposeThe hypothesis that locally-released iloprost, a synthetic prostacyclin analog, affects macrophage phenotype at a microdialysis implant in the subcutaneous space of rats was tested. Macrophage activation towards alternatively-activated phenotypes using pharmaceutical release is of interest to improve integration of implants and direct the foreign body reaction toward a successful outcome.

methodsMacrophage cell culture was used to test iloprost macrophage activation in vitro. Microdialysis sampling probes were implanted into the subcutaneous space of Sprague-Dawley rats to locally deliver iloprost in awake- and freely-moving rats. Monocyte chemoattractant protein -1 (CCL2) was quantified from collected dialysates using ELISA. Immunohistochemical staining was used to determine the presence of CD163

resultsIloprost reduced CCL2 concentrations in NR8383 macrophages stimulated with lipopolysaccharide. CCL2 concentrations in collected dialysates were similarly reduced in the presence of iloprost. Iloprost caused an increase in CD163

conclusionsLocalized delivery of iloprost caused macrophage activation at the tissue interface of a microdialysis subcutaneous implant in rat. This model system may be useful for testing other potential macrophage modulators in vivo.

Indexed as

AnimalsAntigens, CDAntigens, Differentiation, MyelomonocyticCD163 AntigenCell Culture TechniquesCell LineChemokine CCL2Drug LiberationHumansIloprostLipopolysaccharidesMacrophage ActivationMacrophagesMaleMicrodialysisRatsAntigens, CDAntigens, Differentiation, MyelomonocyticCCL2 protein, humanCD163 AntigenChemokine CCL2IloprostLipopolysaccharidesReceptors, Cell SurfaceCCL2 (MCP-1, monocyte chemoattractant protein-1)CD163IloprostMacrophage activationMicrodialysisRat

Identifiers

PMID29305668
OpenAlexW2782034772

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.