Evidence map›Paper›PMID 29303078›Full record

ArticleCurrent gene therapy2018

Molecular Adjuvants Based on Plasmids Encoding Protein Aggregation Domains Affect Bone Marrow Niche Homeostasis.

Maria Giovanna Sabbieti, Giovanna Lacava, Andrea Amaroli, Luigi Marchetti, Roberta Censi, Piera Di Martino, Dimitrios Agas

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current gene therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Maria Giovanna SabbietiSchool of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino (MC), Italy.
Giovanna LacavaSchool of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino (MC), Italy.
Andrea AmaroliDepartment of Surgical and Diagnostic Sciences, University of Genova, Genova, Italy.
Luigi MarchettiSchool of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino (MC), Italy.
Roberta CensiSchool of Pharmacy, University of Camerino, Camerino, (MC), Italy.
Piera Di MartinoSchool of Pharmacy, University of Camerino, Camerino, (MC), Italy.
Dimitrios AgasSchool of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino (MC), Italy.
Università di Camerino · ITUniversity of Genoa · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDuring last years, DNA vaccine immunogenicity has been optimized by the employment of co-stimulatory molecules and molecular adjuvants. It has been reported that plasmid (pATRex), encompassing the DNA sequence for the von Willebrand A (vWA/A) domain of the Anthrax Toxin Receptor-1 (ANTXR-1, alias TEM8, Tumor Endothelial Marker 8), acts as strong immune adjuvant by inducing formation of insoluble intracellular aggregates. Markedly, we faced with upsetting findings regarding the safety of pATRex as adjuvant since the aggregosome formation prompted to osteopenia in mice.

objectiveThe present study provides additional evidences about the proteinaceous adjuvants action within bone marrow and questioned regarding the self-aggregation protein adjuvants immunotoxicity on marrow niches. METHODS &

resultsUsing histological, biochemical and proteomic assays we shed light on pATRex effects within bone marrow niche and specifically we evidenced an aplastic-like bone marrow with disrupted cytokine/chemokine production.

conclusionThe above findings provide compelling support to the thesis that adjuvants based on plasmids encoding protein aggregation domains disrupt the physiological features of the bone marrow elements.

Indexed as

Adjuvants, ImmunologicAnimalsBinding SitesBone Marrow CellsCells, CulturedChemokinesCytokinesDNAFemaleHomeostasisHumansMaleMice, Inbred BALB CPlasmidsProtein Aggregation, PathologicalProteomicsAdjuvants, ImmunologicChemokinesCytokinesDNAAdjuvantsBone marrowBone marrow nicheDNA vaccinesOsteopeniaProtein aggregates.

Identifiers

PMID29303078
PMCPMC6751345
OpenAlexW2781897090

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.