ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2018
Pyramiding of transgenic Pm3 alleles in wheat results in improved powdery mildew resistance in the field.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 72 citations in OpenAlex.
- No Cost of Resistance in Wheat Gene Stack Lines Containing 10 Stem Rust Resistance Transgenes.Plant biotechnology journal · 2026Article
- Dual recognition of structurally unrelated mildew effectors underlies the broad-spectrum resistance of Pm3e in wheat.Nature communications · 2026Article
- QTL Mapping for Leaf Rust Resistance in a Common Wheat Recombinant Inbred Line Population of Doumai/Shi4185.Plants (Basel, Switzerland) · 2025Article
- Discovery of variation in genes related to agronomic traits by sequencing the genome of Cucurbita pepo varieties.BMC genomics · 2025Article
- All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Molecular identification of a Pm4 allele conferring powdery mildew resistance in durum wheat DR88.BMC plant biology · 2024Article
- Genetic basis of an elite wheat cultivar Guinong 29 with harmonious improvement between multiple diseases resistance and other comprehensive traits.Scientific reports · 2024Article
- Pyramiding of transgenic immune receptors from primary and tertiary wheat gene pools improves powdery mildew resistance in the field.Journal of experimental botany · 2024Article
- Breeding for durable resistance against biotrophic fungal pathogens using transgenes from wheat.Molecular breeding : new strategies in plant improvement · 2024Article
- Genome-wide association analysis identifies a consistent QTL for powdery mildew resistance on chromosome 3A in Nordic and Baltic spring wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance.Nature communications · 2024Article
- Exploring the genetic architecture of powdery mildew resistance in wheat through QTL meta-analysis.Frontiers in plant science · 2024Article
- Effects of pathogen reproduction system on the evolutionary and epidemiological control provided by deployment strategies for two major resistance genes in agricultural landscapes.Evolutionary applications · 2024Article
- Haplotype Analysis Sheds Light on the Genetic Evolution of the Powdery Mildew Resistance LocusPathogens (Basel, Switzerland) · 2023Article
- Identification and transfer of a new Pm21 haplotype with high genetic diversity and a special molecular resistance mechanism.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Integrated transcriptome and metabolome analysis reveals that flavonoids function in wheat resistance to powdery mildew.Frontiers in plant science · 2023Article
- Plant and pathogen genomics: essential approaches for stem rust resistance gene stacks in wheat.Frontiers in plant science · 2023Review
- Unmasking the invaders: NLR-mal function in plant defense.Frontiers in plant science · 2023Review
- Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning.Nature genetics · 2022Article
- Identification, High-Density Mapping, and Characterization of New Major Powdery Mildew Resistance Loci From the Emmer Wheat Landrace GZ1.Frontiers in plant science · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
key messageThe combined effects of enhanced total transgene expression level and allele-specificity combination in transgenic allele-pyramided Pm3 wheat lines result in improved powdery mildew field resistance without negative pleiotropic effects. Allelic Pm3 resistance genes of wheat confer race-specific resistance to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) and encode nucleotide-binding domain, leucine-rich repeat (NLR) receptors. Transgenic wheat lines overexpressing alleles Pm3a, b, c, d, f, and g have previously been generated by transformation of cultivar Bobwhite and tested in field trials, revealing varying degrees of powdery mildew resistance conferred by the transgenes. Here, we tested four transgenic lines each carrying two pyramided Pm3 alleles, which were generated by crossbreeding of lines transformed with single Pm3 alleles. All four allele-pyramided lines showed strongly improved powdery mildew resistance in the field compared to their parental lines. The improved resistance results from the two effects of enhanced total transgene expression levels and allele-specificity combinations. In contrast to leaf segment tests on greenhouse-grown seedlings, no allelic suppression was observed in the field. Plant development and yield scores of the pyramided lines were similar to the mean scores of the corresponding parental lines, and thus, the allele pyramiding did not cause any negative effects. On the contrary, in pyramided line, Pm3b × Pm3f normal plant development was restored compared to the delayed development and reduced seed set of parental line Pm3f. Allele-specific RT qPCR revealed additive transgene expression levels of the two Pm3 alleles in the pyramided lines. A positive correlation between total transgene expression level and powdery mildew field resistance was observed. In summary, allele pyramiding of Pm3 transgenes proved to be successful in enhancing powdery mildew field resistance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.