ArticlePeerJ2017
Profiling soil microbial communities with next-generation sequencing: the influence of DNA kit selection and technician technical expertise.
Article in PeerJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 64 citations in OpenAlex.
- Aerobiology and Environmental Zonation in Gypsum Caves: A Comparative Study of Culturing and NGS Approaches.Microbial ecology · 2025Article
- Consistent microbial insights across sequencing methods in soil studies: the role of reference taxonomies.mSystems · 2025Article
- Decoding past microbial life and antibiotic resistance in İnonü Cave's archaeological soil.PloS one · 2025Article
- Enhancing seafood traceability: tracking the origin of seabass and seabream from the tuscan coast area by the analysis of the gill bacterial communities.Animal microbiome · 2024Article
- Cutting edge tools in the field of soil microbiology.Current research in microbial sciences · 2024Review
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- Comparative evaluation of soil DNA extraction kits for long read metagenomic sequencing.Access microbiology · 2024Article
- Soil Microbial Communities Associated with Three Arctic Plants in Different Local Environments in Ny-Ålesund, Svalbard.Journal of microbiology and biotechnology · 2022Article
- Effects of soft rock on soil properties and bacterial community in Mu Us Sandy Land, China.PeerJ · 2022Article
- Article
- Effects of DNA preservation solution and DNA extraction methods on microbial community profiling of soil.Folia microbiologica · 2021Article
- Soil Metabarcoding Offers a New Tool for the Investigation and Hunting of Truffles in Northern Thailand.Journal of fungi (Basel, Switzerland) · 2021Article
- Article
- The Status of Soil Microbiome as Affected by the Application of Phosphorus Biofertilizer: Fertilizer Enriched with Beneficial Bacterial Strains.International journal of molecular sciences · 2020Article
- New Insight into the Composition of Wheat Seed Microbiota.International journal of molecular sciences · 2020Article
- Article
- Discovery of Novel Biosynthetic Gene Cluster Diversity From a Soil Metagenomic Library.Frontiers in microbiology · 2020Article
- The mycobiome of Australian tree hollows in relation to theEcology and evolution · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Structure and diversity of microbial communities are an important research topic in biology, since microbes play essential roles in the ecology of various environments. Different DNA isolation protocols can lead to data bias and can affect results of next-generation sequencing. To evaluate the impact of protocols for DNA isolation from soil samples and also the influence of individual handling of samples, we compared results obtained by two researchers (R and T) using two different DNA extraction kits: (1) MO BIO PowerSoil
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.