Evidence map›Paper›PMID 29301570›Full record

ArticleParasites & vectors2018

MALDI-TOF MS as a new tool for the identification of Dientamoeba fragilis.

Adriana Calderaro, Mirko Buttrini, Sara Montecchini, Sabina Rossi, Giovanna Piccolo, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto

Abstract readEvaluation Study
In one paragraph

Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Journal of medical microbiology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Adriana CalderaroDepartment of Medicine and Surgery, University of Parma, Parma, Italy. adriana.calderaro@unipr.it.ORCID 0000-0002-4308-3120
Mirko ButtriniDepartment of Medicine and Surgery, University of Parma, Parma, Italy.
Sara MontecchiniDepartment of Medicine and Surgery, University of Parma, Parma, Italy.
Sabina RossiDepartment of Medicine and Surgery, University of Parma, Parma, Italy.
Giovanna PiccoloDepartment of Medicine and Surgery, University of Parma, Parma, Italy.
Maria Cristina ArcangelettiDepartment of Medicine and Surgery, University of Parma, Parma, Italy.
Maria Cristina MediciDepartment of Medicine and Surgery, University of Parma, Parma, Italy.
Carlo ChezziDepartment of Medicine and Surgery, University of Parma, Parma, Italy.
Flora De ContoDepartment of Medicine and Surgery, University of Parma, Parma, Italy.

Funding

Bureau of the Council of Ministers - Italian National Committee for Biosafety, Biotechnology and Life Sciences The Biobank of microorganisms and viruses pathogenic to humans as a starting point for the study of the infectious diseases and zoonosesMinistero dell'Istruzione, dell'Università e della Ricerca FIL
6 · The paper itself

Abstract

backgroundIn this study for the first time, a Dientamoeba fragilis protein profile by MALDI-TOF MS was created in order to identify specific markers for the application of this technology in the laboratory diagnosis of dientamoebiasis. In particular, one D. fragilis reference strain was used to create a reference spectrum and 14 clinical isolates to verify the reliability of the obtained results.

resultsWhile 15 peaks were found to be discriminating between the reference strain and the culture medium used, six peaks, observed in all the 14 strains tested, were considered as markers able to identify D. fragilis.

conclusionsIn our hands, MALDI-TOF MS technology was demonstrated as a useful tool to be used in association with or in replacement of the real-time PCR assay for the identification of D. fragilis used in our laboratory on xenic cultures, due to its accuracy, rapidity and low cost.

Indexed as

Costs and Cost AnalysisDientamoebaParasitologyProteomeProtozoan ProteinsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTime FactorsProteomeProtozoan ProteinsDientamoeba fragilisIdentificationMALDI-TOF mass spectrometry

Identifiers

PMID29301570
PMCPMC5755284

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.