ArticleParasites & vectors2018
Culex pipiens pallens cuticular protein CPLCG5 participates in pyrethroid resistance by forming a rigid matrix.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 101 citations in OpenAlex.
- Valorization of Ficus carica Pruning Residues as Selective Botanical Insecticides: Optimized Furanocoumarin Extraction, Efficacy Against Neotropical Stink Bugs, and Mechanistic Insights via Molecular Docking.Archives of insect biochemistry and physiology · 2026Article
- Ecotypes,bioRxiv : the preprint server for biology · 2026Article
- Article
- Identification and Characterization of Cuticular Proteins in the Miridae InsectInternational journal of molecular sciences · 2026Article
- Comparative transcriptomics reveals different profiles between diflubenzuron-resistant and -susceptible phenotypes of the mosquito Culex pipiens.Pest management science · 2025Article
- The dual role of the RR-2 cuticular protein gene in development and acaricide susceptibility of Panonychus citri (Acari: Tetranychidae).Experimental & applied acarology · 2025Article
- A Leg Cuticle Protein Enhances the Resistance ofInternational journal of molecular sciences · 2025Article
- Ultrastructure and Transcriptome Analysis of the Larval Integument in Solitary and Gregarious Phases ofInsects · 2025Article
- Panoramic view of diversity and function of cuticular proteins in insects and mosquitoes biology.Frontiers in insect science · 2025Review
- The impact of agrochemical pollutant mixtures on the selection of insecticide resistance in the malaria vector Anopheles gambiae: insights from experimental evolution and transcriptomics.Malaria journal · 2024Article
- Knockdown ofFrontiers in cellular and infection microbiology · 2024Article
- Cuticular competing endogenous RNAs regulate insecticide penetration and resistance in a major agricultural pest.BMC biology · 2023Article
- A chromosome-level genome assembly of Stenchaetothrips biformis and comparative genomic analysis highlights distinct host adaptations among thrips.Communications biology · 2023Article
- Cuticle Modifications and Over-Expression of the Chitin-Synthase Gene in Diflubenzuron-Resistant Phenotype.Insects · 2022Article
- Genome-Wide Scans and Transcriptomic Analyses Characterize Selective Changes as a Result of Chlorantraniliprole Resistance inInternational journal of molecular sciences · 2022Article
- CPR63 promotes pyrethroid resistance by increasing cuticle thickness in Culex pipiens pallens.Parasites & vectors · 2022Article
- Identification and Functional Analysis of Differentially Expressed Genes in Myzus persicae (Hemiptera: Aphididae) in Response to Trans-anethole.Journal of insect science (Online) · 2022Article
- Transcriptomic analysis of resistance and short-term induction response to pyrethroids, in Anopheles coluzzii legs.BMC genomics · 2021Article
- Article
- Genomic and Transcriptomic Analysis Reveals Cuticular Protein Genes Responding to Different Insecticides in Fall ArmywormInsects · 2021Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
backgroundChemical insecticides have hugely reduced the prevalence of vector-borne diseases around the world, but resistance threatens their continued effectiveness. Despite its importance, cuticle resistance is an under-studied area, and exploring the detailed molecular basis of resistance is critical for implementing suitable resistance management strategies.
methodsWe performed western blotting of cuticular protein CPLCG5 in deltamethrin-susceptible (DS) and laboratory-produced deltamethrin-resistant (DR) strains of Culex pipiens pallens. Immunofluorescence assays using a polyclonal antibody to locate cuticular CPLCG5 in mosquitoes. EM immunohistochemical analysis of the femur segment was used to compare the cuticle in control and CPLCG5-deficient siRNA experimental groups.
resultsThe gene CPLCG5 encodes a cuticle protein that plays an important role in pyrethroid resistance. Based on a prior study, we found that expression of CPLCG5 was higher in the resistant (DR) strain than the susceptible (DS) strain. CPLCG5 transcripts were abundant in white pupae and 1-day-old adults, but expression was dramatically decreased in 3-day-old adults, then remained stable thereafter. Western blotting revealed that the CPLCG5 protein was ~2.2-fold higher in the legs of the DR strain than the DS strain. Immunofluorescence assays revealed CPLCG5 expression in the head, thorax, abdomen, wing, and leg, and expression most abundant in the leg and wing. EM immunohistochemical analysis suggested that the exocuticle thickness of the femur was significantly thinner in the CPLCG5-deficient siCPLCG5 strain (0.717 ± 0.110 μm) than the siNC strain (0.946 ± 0.126 μm). Depletion of CPLCG5 by RNA interference resulted in unorganised laminae and a thinner cuticle.
conclusionsThe results suggest CPLCG5 participates in pyrethroid resistance by forming a rigid matrix and increasing the thickness of the cuticle.
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