ArticleACS synthetic biology2018
Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP
Article in ACS synthetic biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Lighting up yeast: overview of optogenetics in yeast and their applications to yeast biotechnology.FEMS yeast research · 2025Review
- Metabolic Engineering ofInternational journal of molecular sciences · 2024Article
- Improved fluorescent phytochromes for in situ imaging.Scientific reports · 2022Article
- A small and highly sensitive red/far-red optogenetic switch for applications in mammals.Nature biotechnology · 2022Article
- Special Chimeric Antigen Receptor (CAR) Modifications of T Cells: A Review.Frontiers in oncology · 2022Review
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- Building a Simple and Versatile Illumination System for Optogenetic Experiments.Journal of visualized experiments : JoVE · 2021Article
- Visualization and Manipulation of Intracellular Signaling.Advances in experimental medicine and biology · 2021Review
- Red Light Optogenetics in Neuroscience.Frontiers in cellular neuroscience · 2021Review
- Reverse and Forward Engineering Multicellular Structures with Optogenetics.Current opinion in biomedical engineering · 2020Article
- CreLite: An optogenetically controlled Cre/loxP system using red light.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Lights, cytoskeleton, action: Optogenetic control of cell dynamics.Current opinion in cell biology · 2020Review
- Controlling gene expression with light: a multidisciplinary endeavour.Biochemical Society transactions · 2020Review
- PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants.iScience · 2020Article
- Engineering light-controllable CAR T cells for cancer immunotherapy.Science advances · 2020Article
- Optogenetic control of Bacillus subtilis gene expression.Nature communications · 2019Article
- Diverse light responses of cyanobacteria mediated by phytochrome superfamily photoreceptors.Nature reviews. Microbiology · 2019Review
- Cyanobacteriochrome-based photoswitchable adenylyl cyclases (cPACs) for broad spectrum light regulation of cAMP levels in cells.The Journal of biological chemistry · 2018Article
- Bringing Light to Transcription: The Optogenetics Repertoire.Frontiers in genetics · 2018Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
Transplanting metabolic reactions from one species into another has many uses as a research tool with applications ranging from optogenetics to crop production. Ferredoxin (Fd), the enzyme that most often supplies electrons to these reactions, is often overlooked when transplanting enzymes from one species to another because most cells already contain endogenous Fd. However, we have shown that the production of chromophores used in Phytochrome B (PhyB) optogenetics is greatly enhanced in mammalian cells by expressing bacterial and plant Fds with ferredoxin-NADP+ reductases (FNR). We delineated the rate limiting factors and found that the main metabolic precursor, heme, was not the primary limiting factor for producing either the cyanobacterial or plant chromophores, phycocyanobilin or phytochromobilin, respectively. In fact, Fd is limiting, followed by Fd+FNR and finally heme. Using these findings, we optimized the PCB production system and combined it with a tissue penetrating red/far-red sensing PhyB optogenetic gene switch in animal cells. We further characterized this system in several mammalian cell lines using red and far-red light. Importantly, we found that the light-switchable gene system remains active for several hours upon illumination, even with a short light pulse, and requires very small amounts of light for maximal activation. Boosting chromophore production by matching metabolic pathways with specific ferredoxin systems will enable the unparalleled use of the many PhyB optogenetic tools and has broader implications for optimizing synthetic metabolic pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.