ArticleJournal of experimental botany2018
Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 58 citations in OpenAlex.
- CRISPR-enhanced microalgae for carbon sequestration and carotenoid production: opportunities and fundamental constraints.Bioresources and bioprocessing · 2026Review
- Transcriptome Analysis Elucidates the Essential Pathways and Candidate Genes Involved in Chloroplast Development Between True Leaves and Cotyledon in Trichosanthes kirilowii Maxim.Biochemical genetics · 2026Article
- Insights into transcriptomics and metabolic engineering of microalgal systems for enhancing industrial and environmental applications.World journal of microbiology & biotechnology · 2026Review
- CRISPR-Cas Technology TurnsMarine drugs · 2025Review
- Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth.Plant biotechnology journal · 2025Article
- Metabolomics Reveals the Impact of Overexpression of Cytosolic Fructose-1,6-Bisphosphatase on Photosynthesis and Growth inInternational journal of molecular sciences · 2024Article
- Photoautotrophic cultivation of a Chlamydomonas reinhardtii mutant with zeaxanthin as the sole xanthophyll.Biotechnology for biofuels and bioproducts · 2024Article
- Advances in light system engineering across the phototrophic spectrum.Frontiers in plant science · 2024Review
- Advances in Genetic Engineering in Improving Photosynthesis and Microalgal Productivity.International journal of molecular sciences · 2023Review
- LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2021Article
- Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection.Nature communications · 2021Article
- Establishment of a Genome Editing Tool Using CRISPR-Cas9 inInternational journal of molecular sciences · 2021Article
- Article
- Potential and Challenges of Improving Photosynthesis in Algae.Plants (Basel, Switzerland) · 2020Review
- TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii.PloS one · 2020Article
- Site-Specific Gene Knock-Out and On-Site Heterologous Gene Overexpression inFrontiers in plant science · 2020Article
- Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear-encoded light-harvesting chlorophyll- and carotenoid-binding proteins (LHCPs) are imported into the chloroplast and transported across the stroma to thylakoid membrane assembly sites by the chloroplast signal recognition particle (CpSRP) pathway. The LHCP translocation defect (LTD) protein is essential for the delivery of imported LHCPs to the CpSRP pathway in Arabidopsis. However, the function of the LTD protein in Chlamydomonas reinhardtii has not been investigated. Here, we generated a C. reinhardtii ltd (Crltd) knockout mutant by using CRISPR-Cas9, a new target-specific knockout technology. The Crltd1 mutant showed a low chlorophyll content per cell with an unusual increase in appressed thylakoid membranes and enlarged cytosolic vacuoles. Profiling of thylakoid membrane proteins in the Crltd1 mutant showed a more severe reduction in the levels of photosystem I (PSI) core proteins and absence of functional LHCI compared with those of photosystem II, resulting in a much smaller PSI pool size and diminished chlorophyll antenna size. The lack of CrLTD did not prevent photoautotrophic growth of the cells. These results are substantially different from those for Arabidopsis ltd null mutant, indicating LTD function in LHCP delivery and PSI assembly may not be as stringent in C. reinhardtii as it is in higher plants.
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