ArticleAnnals of botany2018
The Arabidopsis thaliana non-specific phospholipase C2 is involved in the response to Pseudomonas syringae attack.
Article in Annals of botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Non-specific phospholipase Cs and their potential for crop improvement.Journal of experimental botany · 2026Review
- The lncRNA LOC100257036 and vvimiR156 modulate gibberellin signaling through AGAMOUS during cluster formation in Sistan Yaghooti grape.Scientific reports · 2025Article
- Low-Cost Heating Modalities Allow the Detection of Biomarkers for Plant Infection Using Rapid Evaporative Ionization Mass Spectrometry (REIMS) That Are Pathogen Specific.Analytical chemistry · 2025Article
- The non-specific phospholipase C of common bean PvNPC4 modulates roots and nodule development.PloS one · 2025Article
- Article
- Nonspecific phospholipases C3 and C4 interact with PIN-FORMED2 to regulate growth and tropic responses in Arabidopsis.The Plant cell · 2024Article
- Phospholipase C: Diverse functions in plant biotic stress resistance and fungal pathogenicity.Molecular plant pathology · 2023Review
- Biophysical and proteomic analyses ofmBio · 2023Article
- The Bro1-like domain-containing protein, AtBro1, modulates growth and abiotic stress responses in Arabidopsis.Frontiers in plant science · 2023Article
- DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis.Plant physiology · 2022Article
- The functions of phospholipases and their hydrolysis products in plant growth, development and stress responses.Progress in lipid research · 2022Review
- Identification of miRNA and their target genes inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Phospholipases C and D and Their Role in Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2021Review
- Characterization of theFrontiers in plant science · 2021Article
- Phospholipase Dα1 Acts as a Negative Regulator of High MgFrontiers in plant science · 2021Article
- Non-specific phospholipase C (NPC): an emerging class of phospholipase C in plant growth and development.Journal of plant research · 2020Review
- Recent Advances in the Cellular and Developmental Biology of Phospholipases in Plants.Frontiers in plant science · 2019Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background and Aims: The non-specific phospholipase C (NPC) is a new member of the plant phospholipase family that reacts to abiotic environmental stresses, such as phosphate deficiency, high salinity, heat and aluminium toxicity, and is involved in root development, silicon distribution and brassinolide signalling. Six NPC genes (NPC1-NPC6) are found in the Arabidopsis genome. The NPC2 isoform has not been experimentally characterized so far. Methods: The Arabidopsis NPC2 isoform was cloned and heterologously expressed in Escherichia coli. NPC2 enzyme activity was determined using fluorescent phosphatidylcholine as a substrate. Tissue expression and subcellular localization were analysed using GUS- and GFP-tagged NPC2. The expression patterns of NPC2 were analysed via quantitative real-time PCR. Independent homozygous transgenic plant lines overexpressing NPC2 under the control of a 35S promoter were generated, and reactive oxygen species were measured using a luminol-based assay. Key Results: The heterologously expressed protein possessed phospholipase C activity, being able to hydrolyse phosphatidylcholine to diacylglycerol. NPC2 tagged with GFP was predominantly localized to the Golgi apparatus in Arabidopsis roots. The level of NPC2 transcript is rapidly altered during plant immune responses and correlates with the activation of multiple layers of the plant defence system. Transcription of NPC2 decreased substantially after plant infiltration with Pseudomonas syringae, flagellin peptide flg22 and salicylic acid treatments and expression of the effector molecule AvrRpm1. The decrease in NPC2 transcript levels correlated with a decrease in NPC2 enzyme activity. NPC2-overexpressing mutants showed higher reactive oxygen species production triggered by flg22. Conclusions: This first experimental characterization of NPC2 provides new insights into the role of the non-specific phospholipase C protein family. The results suggest that NPC2 is involved in the response of Arabidopsis to P. syringae attack.
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